Proteomic profile of root canal contents in teeth with post-treatment endodontic disease

Autor(es)

Francisco, P. A. ^{[1, 2]} ; Delboni, M. G. ^{[2, 3]} ; Lima, A. R. ^[2] ; Xiao, Y. ^[1] ; Siqueira, W. L. ^[1] ; Gomes, B. P. F. A. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Western Ontario, Schulich Sch Med & Dent, Dept Biochem, London, ON - Canada

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, Endodont Div, Dept Restorat Dent, Av Limeira 901, BR-13414903 Piracicaba, SP - Brazil

^[3] DeVry Facid Univ, Coll Dent, Teresina, Piaui - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Endodontic Journal; v. 52, n. 4, p. 451-460, APR 2019.
• Citações Web of Science: 0

Resumo

Aim To characterize the proteome of 20 root canals in teeth with post-treatment endodontic disease using mass spectrometry and to correlate the identified proteins with clinical features. Methodology Twenty patients with radiographic evidence of apical periodontitis and need for root canal re-treatment were selected. Samples from the root canal contents were collected and processed using two-dimensional capillary nano-flow liquid chromatography and electrospray ionization tandem mass spectrometry. The acquired spectra were separately searched against specific protein database. The results obtained were analysed using descriptive statistics. Additionally, Pearson's chi-square test or one-sided Fisher's exact test, as appropriate, was chosen to examine the null hypothesis that there is no relationship between each clinical feature and the presence of specific microbial or human proteins. Significance levels were set at 5% (P < 0.05). Results A total of 1153 human and 720 microbial UniProt accession numbers corresponding to proteins were recovered. The greater prevalence of proteins was related to biological functions, such as cellular and metabolic processes. A considerable number of microbial proteins with clinical relevance functions, such as pathogenesis/virulence, proteolysis, cell adhesion and drug resistance, were detected. Common endodontic pathogens related to post-treatment endodontic disease such as Enterococcus spp., Propionibacterium spp. and Streptococcus spp. were associated with 23, 40 and 94 distinct proteins, respectively. As for human proteins, many factors related to the immune system process were detected. No significant correlations were found between microbial and human proteins and the clinical features investigated (P > 0.05). Conclusions A considerable number of microbial and human proteins were identified using proteomic analyses, being mainly related to processes indicating cell viability. No significant correlation was found between proteins and clinical features. These findings suggest a network of important microbial pathogenic functions that may be responsible for the host immune system response. (AU)

• Processo FAPESP: 17/16516-7 - Investigação do conteúdo microbiológico, expressão de metaloproteinases de matriz e suscetibilidade antimicrobiana de bactérias específicas em casos de insucesso endodôntico
• Beneficiário: Priscila Amanda Francisco
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/23479-5 - Microbiomas e aspectos imunobiológicos nas infecções endodônticas
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/19743-1 - Análise metaproteômica do conteúdo do canal radicular de dentes com insucesso do tratamento endodôntico
• Beneficiário: Priscila Amanda Francisco
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
• Processo FAPESP: 15/19215-2 - Detecção de micro-organismos e de seus fatores de virulência na saliva, câmara pulpar e canal radicular de dentes associados ao insucesso endodôntico
• Beneficiário: Priscila Amanda Francisco
• Modalidade de apoio: Bolsas no Brasil - Mestrado