Action of antimicrobial photodynamic therapy with red leds in microorganisms related to halitose Controlled and randomized clinical trial

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Costa da Mota Ciarcia, Ana Carolina ^[1] ; Leal Goncalves, Marcela Leticia ^[1] ; Ratto Tempestine Horliana, Anna Carolina ^{[1, 2]} ; Ando Suguimoto, Ellen Sayuri ^[3] ; Araujo, Lysianne ^[4] ; Laselva, Andreia ^[4] ; Alves Mayer, Marcia Pinto ^[3] ; Motta, Lara Jansinsk ^{[1, 2]} ; Melo Deana, Alessandro ^{[1, 2]} ; Mesquita-Ferrari, Raquel Agnelli ^{[1, 2]} ; Santos Fernandes, Kristianne Porta ^[1] ; Bussadori, Sandra Kalil ^[1]

Afiliação do(s) autor(es)

^[1] Nove de Julho Univ, Postgrad Program Biophoton Appl Hlth Sci, Vergueiro St, 235-249 Liberdade, BR-01504001 Sao Paulo, SP - Brazil

^[2] Nove de Julho Univ, Postgrad Program Rehabil Sci, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Microbiol ICB, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Sci Initiat Program Biophoton Appl Hlth Sci, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MEDICINE; v. 98, n. 1 JAN 2019.
• Citações Web of Science: 0

Resumo

Introduction: Halitosis is the term used to describe any unpleasant odor relative to expired air regardless of its source. The prevalence of halitosis in the population is approximately 30%, of which 80 to 90% of the cases originate in the oral cavity resulting from proteolytic degradation by gram negative anaerobic bacteria. Antimicrobial photodynamic therapy (aPDT) has been widely used with very satisfactory results in the health sciences. It involves the use of a non-toxic dye, called photosensitizer (FS), and a light source of a specific wavelength in the presence of the environmental oxygen. This interaction is capable of creating toxic species that generate cell death. The objective of this controlled clinical study is to verify the effect of aPDT in the treatment of halitosis by evaluating the formation of volatile sulphur compounds with gas chromatography and microbiological analysis before and after treatment. Materials and Methods: Young adults in the age group between 18 and 25 years with diagnosis of halitosis will be included in this research. The selected subjects will be divided into 3 groups: G1: aPDT; G2: scraper, and G3: aPDT and scraper. All subjects will be submitted to microbiological analysis and evaluation with Oral ChromaTM before, immediately after treatment, 7, 14, and 30 days after treatment. For the evaluation of the association of the categorical variables the Chi-square test and Fisher's Exact Test will be used. To compare the means the student t test and analysis of variance (ANOVA) will be used and to analyse the correlation between the continuous variables the correlation test by Pearson will be applied. In the analyses of the experimental differences in each group the Wilcoxon test will be used. For all analyses a level of significance of 95% (P < .05) will be considered. Discussion: Halitosis treatment is a topic that still needs attention. The results of this trial could support decision-making by clinicians regarding aPDT using aPDT for treating halitosis. (AU)

• Processo FAPESP: 17/19983-5 - Ação da terapia fotodinâmica com led vermelho nos microorganismos relacionados a halitose: ensaio clínico controlado e aleatorizado.
• Beneficiário: Sandra Kalil Bussadori
• Modalidade de apoio: Auxílio à Pesquisa - Regular