Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)

Autor(es)

Bombonato, Juliana Rodrigues ^{[1, 2]} ; Silva Bonatelli, Isabel Aparecida ^[1] ; Rodrigues Silva, Gislaine Angelica ^[1] ; Moraes, Evandro Marsola ^[1] ; Zappi, Daniela Cristina ^[3] ; Taylor, Nigel P. ^[4] ; Franco, Fernando Faria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Ctr Ciencias Humanas & Biol, Dept Biol, Rodovia Joao Leme Santos, SP264 Km 110, BR-18052780 Sorocaba, SP - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Programa Posgrad Biol Comparada, Ribeirao Preto, SP - Brazil

^[3] Museu Paraense Emilio Goeldi, Inst Tecnol Vale, Coord Bot, Belem, Para - Brazil

^[4] Natl Pk Board, Singapore Bot Gardens, Singapore - Singapore

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 42, n. 1, p. 87-94, JAN-MAR 2019.
• Citações Web of Science: 0

Resumo

The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous primers previously developed for the genera Ariocarpus, Echinocactus, Polaskia and Pilosocereus, in four taxa of the genus Cereus: C. fernambucensis subsp. fernambucensis, C. fernambucensis subsp. sericifer, C. jamacaru and C. insularis. Nine microsatellite loci were amplified in Cereus resulting in 35.2% of success in transferability, which is higher than the average rate of 10% reported in the literature for cross-genera transferability in eudicots. The genetic variation in the transferred markers was sufficient to perform standard clustering analysis, indicating each population as a cohesive genetic cluster. Overall, the amount of genetic variation found indicates that the transferred SSR markers might be useful in large-scale population studies within the genus Cereus. (AU)

• Processo FAPESP: 14/25227-0 - Análises Filogenéticas Multilocos aplicadas ao entendimento de relações evolutivas entre espécies de Cereus (Cactaceae; Cereeae)
• Beneficiário: Fernando de Faria Franco
• Linha de fomento: Auxílio à Pesquisa - Regular