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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)

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Author(s):
Bombonato, Juliana Rodrigues [1, 2] ; Silva Bonatelli, Isabel Aparecida [1] ; Rodrigues Silva, Gislaine Angelica [1] ; Moraes, Evandro Marsola [1] ; Zappi, Daniela Cristina [3] ; Taylor, Nigel P. [4] ; Franco, Fernando Faria [1]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Carlos, Ctr Ciencias Humanas & Biol, Dept Biol, Rodovia Joao Leme Santos, SP264 Km 110, BR-18052780 Sorocaba, SP - Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Programa Posgrad Biol Comparada, Ribeirao Preto, SP - Brazil
[3] Museu Paraense Emilio Goeldi, Inst Tecnol Vale, Coord Bot, Belem, Para - Brazil
[4] Natl Pk Board, Singapore Bot Gardens, Singapore - Singapore
Total Affiliations: 4
Document type: Journal article
Source: GENETICS AND MOLECULAR BIOLOGY; v. 42, n. 1, p. 87-94, JAN-MAR 2019.
Web of Science Citations: 0
Abstract

The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous primers previously developed for the genera Ariocarpus, Echinocactus, Polaskia and Pilosocereus, in four taxa of the genus Cereus: C. fernambucensis subsp. fernambucensis, C. fernambucensis subsp. sericifer, C. jamacaru and C. insularis. Nine microsatellite loci were amplified in Cereus resulting in 35.2% of success in transferability, which is higher than the average rate of 10% reported in the literature for cross-genera transferability in eudicots. The genetic variation in the transferred markers was sufficient to perform standard clustering analysis, indicating each population as a cohesive genetic cluster. Overall, the amount of genetic variation found indicates that the transferred SSR markers might be useful in large-scale population studies within the genus Cereus. (AU)

FAPESP's process: 14/25227-0 - Multilocus phylogenetic analysis to recover evolutionary relationships among species of genus Cereus (Cactaceae; Cereeae)
Grantee:Fernando de Faria Franco
Support type: Regular Research Grants