Intravaginal Delivery of Syngonanthus nitens (Bong.) Ruhland Fraction Based on a Nanoemulsion System Applied to Vulvovaginal Candidiasis Treatment

Autor(es):Mostrar menos -

dos Santos Ramos, Matheus Aparecido ^[1] ; da Silva, Patricia Bento ^[2] ; de Toledo, Luciani Gaspar ^[1] ; Oda, Fernando Bombarda ^[3] ; da Silva, Isabel Cristiane ^[1] ; dos Santos, Lourdes Campaner ^[4] ; dos Santos, Andre Gonzaga ^[3] ; Gottardo de Almeida, Margarete Teresa ^[5] ; Pavan, Fernando Rogerio ^[1] ; Chorilli, Marius ^[2] ; Bauab, This Maria ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Biol Sci, Sch Pharmaceut Sci, Campus Araraquara, BR-14800903 Sao Paulo, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Drug & Med, Sch Pharmaceut Sci, Campus Araraquara, BR-14800903 Sao Paulo, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Dept Nat Prod & Toxicol, Sch Pharmaceut Sci, Campus Araraquara, BR-14800903 Sao Paulo, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Dept Organ Chem, Chem Inst Campus Araraquara, BR-14800060 Sao Paulo, SP - Brazil

^[5] Med Sch Sao Jose Rio Preto FAMERP, Dept Infect Dis, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF BIOMEDICAL NANOTECHNOLOGY; v. 15, n. 5, p. 1072-1089, MAY 2019.
• Citações Web of Science: 0

Resumo

In this study, was evaluated the chemical composition of a fraction from Syngonanthus nitens extract and its antimicrobial potential unloaded (Fr3) and loaded (F9Fr3) into a nanoemulsion (F9) composed of cholesterol as the oil phase (10%), polyoxyethylene 20-cetyl ether and soy phosphatidylcholine (2:1) as surfactant (20%), and a solution of phosphate buffer (pH 7.4) plus chitosan polymer dispersion (0.25%) as the aqueous phase (70%) to use for VVC treatment. Phytochemical procedures showed that Fr3 is rich in luteolin, which is responsible for the antimicrobial activity. F9 development showed satisfactory parameters for use in the vulvovaginal candidiasis (VVC) treatment, as F9 demonstrated pseudoplastic, elastic behavior, and adhesive properties on vaginal mucosa. In addition, we observed improvement in antimicrobial potential of Fr3 on planktonic and biofilms after incorporation in F9. Fr3 and F9Fr3 showed satisfactory parameters related to toxic profiles in cell lines and in a model of acute toxicity by Artemia salina. The in vivo VVC assay showed that F9Fr3 was more active than unloaded Fr3 in VVC treatment. In conclusion, this work showed that use of a fraction rich in luteolin can be a used as an antimicrobial for treatment of vaginal infections and that use of nanostructured lipid systems was an important factor in the biological efficacy of Fr3, especially in treatment of acute VVC. (AU)

• Processo FAPESP: 18/21626-9 - Liberação intravaginal de uma nanomeulsão contendo fração de Syngonanthus nitens aplicado ao tratamento da candidíase vulvovaginal
• Beneficiário: Taís Maria Bauab
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo
• Processo FAPESP: 14/24626-9 - Syngonanthus nitens (Bong.) Ruhland: prospecção químico-biológica do extrato metanólico de escapos no tratamento da candidíase vulvovaginal.
• Beneficiário: Matheus Aparecido dos Santos Ramos
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/08559-5 - Análise química e prospecção terapêutica de Syngonanthus nitens (Bong.) Ruhland e Cymbopogon nardus (L.) Rendle incorporados em sistemas nanotecnológicos aplicados ao tratamento da candidíase vulvovaginal
• Beneficiário: Taís Maria Bauab
• Modalidade de apoio: Auxílio à Pesquisa - Regular