Intratumoral collagen index predicts mortality and survival in canine cutaneous mast cell tumours

Autor(es)

Daniel, Jessika ^[1] ; Barra, Camila N. ^[2] ; Pulz, Lidia H. ^[2] ; Kleeb, Silvia Regina ^[3] ; Xavier, Jose Guilherme ^[4] ; Catao-Dias, Jose Luiz ^[2] ; Nishiya, Adriana T. ^[5] ; Fukumasu, Heidge ^[1] ; Strefezzi, Ricardo F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Med Vet, LOCT, Fac Zootecnia & Engn Alimentos, Av Duque Caxias Norte 255, BR-13635900 Pirassununga - Brazil

^[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, Av Prof Dr Orlando Marques Paiva 81, BR-05508270 Sao Paulo - Brazil

^[3] Univ Metodista Sao Paulo, Av Dom Jaime De Barros Camara 1000, BR-09895400 Sao Bernardo Do Campo - Brazil

^[4] Univ Paulista, R Dr Bacelar 1212, BR-04026002 Sao Paulo - Brazil

^[5] Univ Anhembi Morumbi, R Dr Almeida Lima 1134, BR-03164 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VETERINARY DERMATOLOGY; v. 30, n. 2, p. 162+, APR 2019.
• Citações Web of Science: 0

Resumo

Background Mast cell tumours (MCTs) constitute almost 25% of cutaneous neoplasms in dogs. Their biological behaviour is predicted using histopathological grading which is based on several subjective criteria that are vulnerable to intra- and interobserver variability. To improve the prediction of the biological behaviour, several complementary markers have been studied. The integrity of the extracellular matrix (ECM) may play a protective role against tumoral progression, and favour cellular proliferation, angiogenesis, invasion and metastases when altered. Hypothesis/Objectives To evaluate the quantification of collagen and elastic fibres as prognostic markers for MCTs. Animals Thirty-eight random cases of canine cutaneous MCT surgically treated with wide margins were included. Methods and materials Intratumoral collagen and elastic fibres were identified and quantified on histological sections stained with Masson's trichrome, Picrosirius red and Verhoeff; the results were compared with histopathological grades, mortality due to the disease and postsurgical survival. Results Morphometric analysis revealed a significant relationship between histopathological grade and intratumoral collagen index (CoI). In addition, the CoI was considered an independent indicator for mortality and postsurgical survival. Conclusions and clinical importance These results support the importance of the CoI in the grading and prognosis of MCTs, suggesting that preservation and/or synthesis of collagen have the potential to become targets for MCT therapeutics. (AU)

• Processo FAPESP: 13/13252-8 - Caracterização de genes relacionados à apoptose em mastocitomas cutâneos caninos e seu valor como indicador prognóstico
• Beneficiário: Ricardo de Francisco Strefezzi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/03862-1 - Caracterização de fibroblastos associados ao câncer e sua influência sobre neoplasias caninas
• Beneficiário: Ricardo de Francisco Strefezzi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/05094-5 - Avaliação imuno-histoquímica das expressões de VEGF, VEGFR e metaloproteinase 9 como potenciais indicadores prognósticos para mastocitomas cutâneos caninos
• Beneficiário: Ricardo de Francisco Strefezzi
• Modalidade de apoio: Auxílio à Pesquisa - Regular