Recombinant RGD-disintegrin DisBa-01 blocks integrin alpha(v)beta(3) and impairs VEGF signaling in endothelial cells

Autor(es)

Danilucci, Tais M. ^[1] ; Santos, Patty K. ^[1] ; Pachane, Bianca C. ^[1] ; Pisani, Graziele F. D. ^[1] ; Lino, Rafael L. B. ^[1] ; Casali, Bruna C. ^[1] ; Altei, Wanessa F. ^[1] ; Selistre-de-Araujo, Heloisa S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Ciencias Fisiol, Ctr Ciencias Biol & Saude, Rod Washington Luis, Km 235 SP-310, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CELL COMMUNICATION AND SIGNALING; v. 17, MAR 20 2019.
• Citações Web of Science: 1

Resumo

BackgroundIntegrins mediate cell adhesion, migration, and survival by connecting the intracellular machinery with the surrounding extracellular matrix. Previous studies demonstrated the interaction between (v3) integrin and VEGF type 2 receptor (VEGFR2) in VEGF-induced angiogenesis. DisBa-01, a recombinant His-tag fusion, RGD-disintegrin from Bothrops alternatus snake venom, binds to (v3) integrin with nanomolar affinity blocking cell adhesion to the extracellular matrix. Here we present in vitro evidence of a direct interference of DisBa-01 with (v3)/VEGFR2 cross-talk and its downstream pathways.MethodsHuman umbilical vein (HUVECs) were cultured in plates coated with fibronectin (FN) or vitronectin (VN) and tested for migration, invasion and proliferation assays in the presence of VEGF, DisBa-01 (1000nM) or VEGF and DisBa-01 simultaneously. Phosphorylation of (v3)/VEGFR2 receptors and the activation of intracellular signaling pathways were analyzed by western blotting. Morphological alterations were observed and quantified by fluorescence confocal microscopy.ResultsDisBa-01 treatment of endothelial cells inhibited critical steps of VEGF-mediated angiogenesis such as migration, invasion and tubulogenesis. The blockage of (v3)/VEGFR2 cross-talk by this disintegrin decreases protein expression and phosphorylation of VEGFR2 and (3) integrin subunit, regulates FAK/SrC/Paxillin downstream signals, and inhibits ERK1/2 and PI3K pathways. These events result in actin re-organization and inhibition of HUVEC migration and adhesion. Labelled-DisBa-01 colocalizes with (v3) integrin and VEGFR2 in treated cells.ConclusionsDisintegrin inhibition of (v3) integrin blocks VEGFR2 signalling, even in the presence of VEGF, which impairs the angiogenic mechanism. These results improve our understanding concerning the mechanisms of pharmacological inhibition of angiogenesis. (AU)

• Processo FAPESP: 13/00798-2 - A matriz extracelular no envelhecimento, no exercício e no microambiente tumoral
• Beneficiário: Heloisa Sobreiro Selistre de Araújo
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/18747-8 - Exossomos de células tumorais de mama e seu papel na adesão celular durante o processo de metástase: estudos in vitro
• Beneficiário: Wanessa Fernanda Altei
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado