Substrate and inhibitor selectivity, and biological activity of an epoxide hydrolase from Trichoderma reesei

Autor(es)

de Oliveira, Gabriel S. ^[1] ; Adriani, Patricia P. ^[1] ; Wu, Hao ^{[2, 3]} ; Morisseau, Christophe ^{[2, 3]} ; Hammock, Bruce D. ^{[2, 3]} ; Chambergo, Felipe S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Artes Ciencias & Humanidades, 1000 Ave Arlindo Bettio, BR-03828000 Sao Paulo - Brazil

^[2] Univ Calif Davis, Dept Entomol & Nematol, One Shields Ave, Davis, CA 95616 - USA

^[3] Univ Calif Davis, UC Davis Comprehens Canc Ctr, One Shields Ave, Davis, CA 95616 - USA

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR BIOLOGY REPORTS; v. 46, n. 1, p. 371-379, FEB 2019.
• Citações Web of Science: 0

Resumo

Epoxide hydrolases (EHs) are present in all living organisms and catalyze the hydrolysis of epoxides to the corresponding vicinal diols. EH are involved in the metabolism of endogenous and exogenous epoxides, and thus have application in pharmacology and biotechnology. In this work, we describe the substrates and inhibitors selectivity of an epoxide hydrolase recently cloned from the filamentous fungus Trichoderma reesei QM9414 (TrEH). We also studied the TrEH urea-based inhibitors effects in the fungal growth. TrEH showed high activity on radioative and fluorescent surrogate and natural substrates, especially epoxides from docosahexaenoic acid. Using a fluorescent surrogate substrate, potent inhibitors of TrEH were identified. Interestingly, one of the best compounds inhibit up to 60% of T. reesei growth, indicating an endogenous role for TrEH. These data make TrEH very attractive for future studies about fungal metabolism of fatty acids and possible development of novel drugs for human diseases. (AU)

• Processo FAPESP: 16/12859-4 - Identificação de substratos e inibidores de epóxido hidrolases de Trichoderma reesei
• Beneficiário: Gabriel Stephani de Oliveira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto
• Processo FAPESP: 15/03329-9 - Clonagem e caracterização de enzimas epóxido hidrolases de Trichoderma reesei
• Beneficiário: Gabriel Stephani de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 14/24107-1 - Estudo e caracterização de enzimas oxidases produzidas por fungos filamentosos
• Beneficiário: Felipe Santiago Chambergo Alcalde
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 17/25705-8 - Estudo e caracterização de enzimas para degradação de biomassa
• Beneficiário: Felipe Santiago Chambergo Alcalde
• Modalidade de apoio: Auxílio à Pesquisa - Regular