Screening of substrates and inhibitors for epoxide hydrolase from Trichoderma reesei
| Full text | |
| Author(s): |
de Oliveira, Gabriel S.
[1]
;
Adriani, Patricia P.
[1]
;
Wu, Hao
[2, 3]
;
Morisseau, Christophe
[2, 3]
;
Hammock, Bruce D.
[2, 3]
;
Chambergo, Felipe S.
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Escola Artes Ciencias & Humanidades, 1000 Ave Arlindo Bettio, BR-03828000 Sao Paulo - Brazil
[2] Univ Calif Davis, Dept Entomol & Nematol, One Shields Ave, Davis, CA 95616 - USA
[3] Univ Calif Davis, UC Davis Comprehens Canc Ctr, One Shields Ave, Davis, CA 95616 - USA
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | MOLECULAR BIOLOGY REPORTS; v. 46, n. 1, p. 371-379, FEB 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Epoxide hydrolases (EHs) are present in all living organisms and catalyze the hydrolysis of epoxides to the corresponding vicinal diols. EH are involved in the metabolism of endogenous and exogenous epoxides, and thus have application in pharmacology and biotechnology. In this work, we describe the substrates and inhibitors selectivity of an epoxide hydrolase recently cloned from the filamentous fungus Trichoderma reesei QM9414 (TrEH). We also studied the TrEH urea-based inhibitors effects in the fungal growth. TrEH showed high activity on radioative and fluorescent surrogate and natural substrates, especially epoxides from docosahexaenoic acid. Using a fluorescent surrogate substrate, potent inhibitors of TrEH were identified. Interestingly, one of the best compounds inhibit up to 60% of T. reesei growth, indicating an endogenous role for TrEH. These data make TrEH very attractive for future studies about fungal metabolism of fatty acids and possible development of novel drugs for human diseases. (AU) | |
| FAPESP's process: | 16/12859-4 - Screening of substrates and inhibitors for epoxide hydrolase from Trichoderma reesei |
| Grantee: | Gabriel Stephani de Oliveira |
| Support type: | Scholarships abroad - Research Internship - Doctorate (Direct) |
| FAPESP's process: | 15/03329-9 - Cloning and characterization of the enzymes epoxide hydrolases from Trichoderma reesei |
| Grantee: | Gabriel Stephani de Oliveira |
| Support type: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 14/24107-1 - Characterization and study of oxidases enzymes from filamentous fungi |
| Grantee: | Felipe Santiago Chambergo Alcalde |
| Support type: | Program for Research on Bioenergy (BIOEN) - Regular Program Grants |
| FAPESP's process: | 17/25705-8 - Characterization and study of enzymes to biomass degradation |
| Grantee: | Felipe Santiago Chambergo Alcalde |
| Support type: | Regular Research Grants |