Liver proteomics unravel the metabolic pathways related to Feed Efficiency in beef cattle

Autor(es):Mostrar menos -

Fonseca, Leydiana D. ^[1] ; Eler, Joanir P. ^[1] ; Pereira, Mikaele A. ^[1] ; Rosa, Alessandra F. ^[1] ; Alexandre, Pamela A. ^[1] ; Moncau, Cristina T. ^[1] ; Salvato, Fernanda ^[2] ; Rosa-Fernandes, Livia ^[3] ; Palmisano, Giuseppe ^[3] ; Ferraz, Jose B. S. ^[1] ; Fukumasu, Heidge ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Anim Sci & Food Engn, Dept Vet Med, BR-13635900 Pirassununga - Brazil

^[2] Univ Estadual Campinas, Inst Biol, BR-13083862 Campinas, SP - Brazil

^[3] Univ Sao Paulo, Biomed Sci Inst, Dept Parasitol, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, MAR 29 2019.
• Citações Web of Science: 0

Resumo

Improving nutrient utilization efficiency is essential for livestock, given the current scenario of increasing demand for animal protein and sustainable resource use. In this context, understanding the biology of feed efficiency (FE) in beef cattle allows the development of markers for identification and selection of best animals for animal production. Thus, 98 young Nellore bulls were evaluated for FE and at the end of the experiment liver samples from six High Feed Efficient (HFE) and six Low Feed Efficient (LFE) animals were collected for protein extraction, digestion and analysis by HPLC-MS/MS. Data were analyzed for differential abundant proteins (DAPs), protein networks, and functional enrichment. Serum endotoxin was also quantified. We found 42 DAPs and 3 protein networks significantly related to FE. The main pathways associated with FE were: microbial metabolism; biosynthesis of fatty acids, amino acids and vitamins; glycolysis/gluconeogenesis; xenobiotic metabolism and; antigen processing and presentation. Serum endotoxins were significantly higher in LFE animals supporting the results. Therefore, the findings presented here confirmed the altered hepatic metabolism and pronounced hepatic inflammation in LFE animals supporting that the increased bacterial load is at least in part responsible for the hepatic lesions and inflammation in LFE animals. (AU)

• Processo FAPESP: 14/04937-0 - Análise proteômica comparativa de fígado de bovinos Nelore machos de alta e baixa eficiência alimentar
• Beneficiário: Leydiana Duarte Fonseca
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/12492-8 - Utilização da genômica e proteômica para avaliação do efeito do estresse pré-abate na qualidade da carne de bovinos cruzados terminados em confinamento
• Beneficiário: Joanir Pereira Eler
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/07566-2 - Genômica aplicada à produção de ruminantes
• Beneficiário: José Bento Sterman Ferraz
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/02493-7 - Neoplasias mamárias de cadelas e a teoria das células-tronco cancerosas: uma abordagem comparada e translacional
• Beneficiário: Heidge Fukumasu
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores