Liver proteomics unravel the metabolic pathways related to Feed Efficiency in beef cattle

Author(s):Show less -

Fonseca, Leydiana D. ^[1] ; Eler, Joanir P. ^[1] ; Pereira, Mikaele A. ^[1] ; Rosa, Alessandra F. ^[1] ; Alexandre, Pamela A. ^[1] ; Moncau, Cristina T. ^[1] ; Salvato, Fernanda ^[2] ; Rosa-Fernandes, Livia ^[3] ; Palmisano, Giuseppe ^[3] ; Ferraz, Jose B. S. ^[1] ; Fukumasu, Heidge ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Anim Sci & Food Engn, Dept Vet Med, BR-13635900 Pirassununga - Brazil

^[2] Univ Estadual Campinas, Inst Biol, BR-13083862 Campinas, SP - Brazil

^[3] Univ Sao Paulo, Biomed Sci Inst, Dept Parasitol, BR-05508900 Sao Paulo - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 9, MAR 29 2019.
• Web of Science Citations: 0

Abstract

Improving nutrient utilization efficiency is essential for livestock, given the current scenario of increasing demand for animal protein and sustainable resource use. In this context, understanding the biology of feed efficiency (FE) in beef cattle allows the development of markers for identification and selection of best animals for animal production. Thus, 98 young Nellore bulls were evaluated for FE and at the end of the experiment liver samples from six High Feed Efficient (HFE) and six Low Feed Efficient (LFE) animals were collected for protein extraction, digestion and analysis by HPLC-MS/MS. Data were analyzed for differential abundant proteins (DAPs), protein networks, and functional enrichment. Serum endotoxin was also quantified. We found 42 DAPs and 3 protein networks significantly related to FE. The main pathways associated with FE were: microbial metabolism; biosynthesis of fatty acids, amino acids and vitamins; glycolysis/gluconeogenesis; xenobiotic metabolism and; antigen processing and presentation. Serum endotoxins were significantly higher in LFE animals supporting the results. Therefore, the findings presented here confirmed the altered hepatic metabolism and pronounced hepatic inflammation in LFE animals supporting that the increased bacterial load is at least in part responsible for the hepatic lesions and inflammation in LFE animals. (AU)

• FAPESP's process: 14/04937-0 - Comparative proteomic analysis of liver Nelore cattle males of high and low feed efficiency
• Grantee: Leydiana Duarte Fonseca
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/12492-8 - Using genomics and proteomics to assess the pre-slaughter stress effect on the meat quality of crossbred cattle finished in feedlot
• Grantee: Joanir Pereira Eler
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/07566-2 - Genomics applied to ruminant production
• Grantee: José Bento Sterman Ferraz
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/02493-7 - Mammary tumors of dogs and the cancer stem cell theory: a comparative and translational approach
• Grantee: Heidge Fukumasu
• Support Opportunities: Research Grants - Young Investigators Grants