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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Liposome-Based Biosensors Using Phytase Immobilized on Polypyrrole Films for Phytic Acid Determination

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Autor(es):
Rodrigues, Valquiria C. [1] ; Moraes, Marli L. [2] ; Soares, Juliana C. [1] ; Souza, Adriano L. [3] ; Soares, Andrey C. [2] ; Oliveira Jr, Osvaldo N. ; Goncalves, Debora [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Fed Sao Paulo, Inst Sci & Technol, Sao Jose Dos Campos, SP - Brazil
[3] Univ Fed Sao Carlos, Rod Anhanguera Km 174, POB 153, BR-13604900 Araras, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN; v. 92, n. 4, p. 847-851, APR 2019.
Citações Web of Science: 0
Resumo

Phytase was immobilized onto electrochemically prepared polypyrrole (PPy) films either in the free, native form (Phy) or encapsulated in phospholipid dipalmitoyl phosphatidylglycerol (DPPG) liposomes (lipo-Phy). The incorporation of Phy into the PPy matrix was verified using cyclic voltammetry, polarization-modulated infrared reflection absorption spectroscopy, and scanning electron microscopy. PPy, PPy/Phy and PPy/lipo-Phy films were characterized by amperometric experiments in phytic acid (PA) solutions at different concentrations. PPy/lipo-Phy showed a more sensitive response, which was confirmed in stable current vs concentration and current vs time curves, and by a superior analytical performance with a saturation concentration of 3.0 mmol L-1 PA. A multidimensional projection technique, IDMAP, was used to project the voltammetric data, and confirmed the higher selectivity for PPy/lipo-Phy by distinguishing PA in a specific range of concentrations. (AU)

Processo FAPESP: 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico
Beneficiário:Osvaldo Novais de Oliveira Junior
Linha de fomento: Auxílio à Pesquisa - Temático