Liposome-Based Biosensors Using Phytase Immobilized on Polypyrrole Films for Phytic Acid Determination

Author(s)

Rodrigues, Valquiria C. ^[1] ; Moraes, Marli L. ^[2] ; Soares, Juliana C. ^[1] ; Souza, Adriano L. ^[3] ; Soares, Andrey C. ^[2] ; Oliveira Jr, Osvaldo N. ; Goncalves, Debora ^[1]

Affiliation

^[1] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Paulo, Inst Sci & Technol, Sao Jose Dos Campos, SP - Brazil

^[3] Univ Fed Sao Carlos, Rod Anhanguera Km 174, POB 153, BR-13604900 Araras, SP - Brazil

• Document type: Journal article
• Source: BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN; v. 92, n. 4, p. 847-851, APR 2019.
• Web of Science Citations: 1

Abstract

Phytase was immobilized onto electrochemically prepared polypyrrole (PPy) films either in the free, native form (Phy) or encapsulated in phospholipid dipalmitoyl phosphatidylglycerol (DPPG) liposomes (lipo-Phy). The incorporation of Phy into the PPy matrix was verified using cyclic voltammetry, polarization-modulated infrared reflection absorption spectroscopy, and scanning electron microscopy. PPy, PPy/Phy and PPy/lipo-Phy films were characterized by amperometric experiments in phytic acid (PA) solutions at different concentrations. PPy/lipo-Phy showed a more sensitive response, which was confirmed in stable current vs concentration and current vs time curves, and by a superior analytical performance with a saturation concentration of 3.0 mmol L-1 PA. A multidimensional projection technique, IDMAP, was used to project the voltammetric data, and confirmed the higher selectivity for PPy/lipo-Phy by distinguishing PA in a specific range of concentrations. (AU)

• FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants