Reappraisal of antibodies against Saccharomyces cerevisiae (ASCA) as persistent biomarkers in quiescent Crohn's disease

Autor(es)

Duarte-Silva, Murillo ^{[1, 2]} ; Afonso, Poliana Cristina ^[3] ; de Souza, Patricia Reis ^{[3, 1]} ; Peghini, Bethanea Crema ^[3] ; Rodrigues-Junior, Virmondes ^[3] ; de Barros Cardoso, Cristina Ribeiro ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, Ribeirao Preto, SP - Brazil

^[3] Univ Fed Triangulo Mineiro, Inst Ciencias Biol & Nat, Uberaba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AUTOIMMUNITY; v. 52, n. 1, p. 37-47, JAN 2 2019.
• Citações Web of Science: 1

Resumo

A clear correlation exists between microbiota and the dysregulation of the immune response in Inflammatory Bowel Diseases (IBD), which comprise Crohn's disease (CD) and ulcerative colitis (UC). These unbalanced reactions also involve humoral responses, with antibodies against Saccharomyces cerevisiae. Thus, here we aimed to quantify IgA and IgG specific to S. cerevisiae (ASCA) in quiescent CD and UC, to correlate the production of these antibodies with patient's inflammatory response and disease clinical presentation. Twenty-nine subjects (16CD and 13 UC) and 45 healthy controls were enrolled in this study and had plasma samples tested for ASCA and cytokines (IL-2, IL-4, IL-6, IL-10, IFN-, TNF-), besides clinical evaluation. IBD patients had increase IgA and IgG ASCA, especially those with colonic (L2) and fistulizing (B3) CD. Similarly, patients who dropped out the treatment had augmented ASCA, while IgG was reduced in those receiving sulfasalazine treatment. Furthermore, the quiescent CD patients had elevated IL-6 on plasma, especially in the absence of treatment, together with increased counter regulatory response of IL-10. There was a positive correlation between IgA and IgG on CD but not UC, as well as between IgA and TNF in total IBD patients. In addition, the levels of IgG x TNF, IgA x IL-10 and IgG x IL-10 were also correlated in CD, indicating that ASCA production may be influenced by the inflammatory response. Finally, we concluded that ASCA could be pointed as relevant biomarker of CD presentation and residual inflammation, even in clinical remission patients. (AU)

• Processo FAPESP: 10/20162-7 - Papel do eixo hipotálamo-pituitária adrenal e glicocorticóides exógenos na modulação da resposta imune na doença inflamatória intestinal
• Beneficiário: Cristina Ribeiro de Barros Cardoso
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores