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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Temporal Change of Extracellular Matrix during Vein Arterialization Remodeling in Rats

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Autor(es):
Miyakawa, Ayumi Aurea [1] ; Bassaneze, Vinicius [1] ; Duarte, Nubia Esteban [1, 2] ; Girao-Silva, Thais [1] ; Bizerra, Monica Nunes [1] ; Campos, Julliana Carvalho [1] ; Krieger, Jose Eduardo [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Med Sch, Lab Genet & Mol Cardiol LIM 13, Heart Inst InCor, BR-05403000 Sao Paulo - Brazil
[2] Univ Nacl Colombia, Dept Matemat, Sede Manizales, Manizales 170003 - Colombia
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE; v. 6, n. 1 MAR 2019.
Citações Web of Science: 0
Resumo

The global expression profile of the arterialized rat jugular vein was established to identify candidate genes and cellular pathways underlying the remodeling process. The arterialized jugular vein was analyzed on days 3 and 28 post-surgery and compared with the normal jugular vein and carotid artery. A gene array platform detected 9846 genes in all samples. A heatmap analysis uncovered patterns of gene expression showing that the arterialized vein underwent a partial transition from vein to artery from day 3 to 28 post-surgery. The same pattern was verified for 1845 key differentially expressed genes by performing a pairwise comparison of the jugular vein with the other groups. Interestingly, hierarchical clustering of 60 genes with altered expression on day 3 and day 28 displayed an expression pattern similar to that of the carotid artery. Enrichment analysis results and the network relationship among genes modulated during vein arterialization showed that collagen might play a role in the early remodeling process. Indeed, the total collagen content was increased, with the augmented expression of collagen I, collagen IV, and collagen V in arterialized veins. Additionally, there was an increase in the expression of versican and Thy-1 and a decrease in the expression of biglycan and beta 1-integrin. Overall, we provide evidence that vein arterialization remodeling is accompanied by consistent patterns of gene expression and that collagen may be an essential element underlying extracellular matrix changes that support the increased vascular wall stress of the new hemodynamic environment. (AU)

Processo FAPESP: 17/05829-4 - Avaliação da influência de fibroblastos sobre a maturação e indução de cardiomiócitos com potencial arritmogênico
Beneficiário:Agatha Ribeiro da Silva
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 13/17368-0 - Genômica cardiovascular: mechanismos & novas terapias - CVGen mech2ther
Beneficiário:José Eduardo Krieger
Linha de fomento: Auxílio à Pesquisa - Temático