Impact of green tea epigallocatechin-3-gallate on HIF1-alpha and mTORC2 expression in obese women: anti-cancer and anti-obesity effects?

Autor(es)

Nicoletti, Carolina Ferreira ^[1] ; Pereira Delfino, Heitor Bernardes ^[1] ; Souza Pinhel, Marcela Augusta ^[1] ; Noronha, Natalia Yumi ^[1] ; Pinhanelli, Vitor Caressato ^[1] ; Gomes Quinhoneiro, Driele Cristina ^[1] ; Parenti Oliveira, Bruno Affonso ^[1] ; Marchini, Julio Sergio ^[1] ; Nonino, Carla Barbosa ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Internal Med, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NUTRICION HOSPITALARIA; v. 36, n. 2, p. 315-320, MAR-APR 2019.
• Citações Web of Science: 0

Resumo

Introduction: epigallocatechin-3-gallate (EGCG) is the most abundant catechin contained in green tea (Camellia sinensis) and has been associated with anti-obesity and anti-cancer effects, but the exact molecular mechanisms remain elusive. In this context, this study was designed to improve the understanding of the EGCG anti-obesity and anti-cancer action. Objectives: this study was designed to examine the effects of EGCG on the expression of genes involved in obesity and cancer pathways in the peripheral blood mononuclear cells of obese women. Material and methods: this longitudinal interventional study enrolled eleven women with severe obesity that were submitted to eight weeks of green tea (decaffeinated green tea capsules with 450.7 mg of EGCG, two capsules/day) supplementation (intervention group) and ten eutrophic women as a control group. Weight (kg), body mass index (BMI, kg/m(2)), fat mass (kg) and gene expression (qPCR method) were assessed before and after supplementation. HIF1-alpha (HIF1-a), phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) and rapamycin-insensitive companion of mTOR (RICTOR) were selected as potential targets. Results: after supplementation, body weight (114.9 +/- 14.3 versus 115 +/- 13.8 kg), body mass index (44.1 +/- 3.7 versus 44.1 +/- 3.9 kg/m(2)) and fat mass (47.6 +/- 3.3 versus 47.3 +/- 3.4 kg) did not change. EGCG upregulated the RICTOR and HIF1-a expression, however, did not modify PI3K expression. Conclusion: this study demonstrated that EGCG has a potential role to obesity and cancer related to obesity control and can be used not only for the purpose of weight loss, but also for the improvement of obesity-related comorbidities. (AU)

• Processo FAPESP: 13/08916-4 - Análise da expressão de genes relacionados ao gasto energético e metabolismo de lipídios de indivíduos com obesidade grau III antes e após intervenções para perda de peso
• Beneficiário: Carla Barbosa Nonino
• Modalidade de apoio: Auxílio à Pesquisa - Regular