Characterization of Intracellular Peptides from Zebrafish (Danio rerio) Brain

Autor(es)

Manzi Teixeira, Caio Matheus ^[1] ; Correa, Claudia Neves ^[1] ; Iwai, Leo Kei ^[2] ; Ferro, Emer Suavinho ^[3] ; de Castro, Leandro Mantovani ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Biosci Inst, Praca Infante Dom Henrique S-N, Sala 4, BR-11330900 Sao Vicente, SP - Brazil

^[2] Butantan Inst, Special Lab Appl Toxinol, Ctr Toxins Immune Response & Cell Signaling, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Pharmacol, Inst Biomed Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ZEBRAFISH; v. 16, n. 3, p. 1-12, APR 2019.
• Citações Web of Science: 4

Resumo

Peptides represent a large class of cell signaling molecules, and they are mainly produced by the classical secretory pathway or during protein degradation. The peptide profile of Danio rerio (zebrafish) shows a lack of information when compared with other consolidated animal models. The aim of this work was to characterize the peptide profile of zebrafish brain by using triplex reductive methylation of amines labeling and liquid chromatography coupled to electron spray mass spectrometry. A total of 411 peptide fragments were detected and 125 peptide sequences could be solved. Further analysis suggested that most of the peptides were fragments of intracellular cytosolic and mitochondrial proteins, and that 60% of the precursor proteins were cleaved at either their N- or C-terminal. The most common residue in the P1 position was leucine whereas other common residues were lysine, alanine, arginine, and phenylalanine. Rare cleavage sites at P1 position were histidine, glutamic acid, and isoleucine. The peptide profile of zebrafish brain has similarities with results previously described in mice brain peptidome studies. Thus, this study represents an important basis for the molecular understanding of zebrafish and its use as a model for human diseases. (AU)

• Processo FAPESP: 16/04000-3 - Farmacologia de oligopeptidases e peptídeos intracelulares
• Beneficiário: Emer Suavinho Ferro
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/22752-0 - Bioprospecção de peptídeos da fauna acompanhante utilizando um ensaio de captura de substrato
• Beneficiário: Leandro Mantovani de Castro
• Modalidade de apoio: Auxílio à Pesquisa - Regular