Differences in embryonic gene expression and quality indicate the benefit of epidermal growth factor receptor inhibitor during prematuration to improve competence in bovine oocytes

Autor(es)

Dall'Acqua, Priscila Chediek ^{[1, 2]} ; Nunes, Giovana Barros ^{[1, 2]} ; da Silva, Cintia Rodrigues ^[2] ; Fontes, Patricia Kubo ^[3] ; Gouveia Nogueira, Marcelo Fabio ^{[3, 4]} ; Lopes, Flavia Lombardi ^[2] ; Marinho, Marcia ^[2] ; Mingoti, Gisele Zoccal ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Dept Prevent Med & Anim Reprod, Sch Agr & Vet Sci, Jaboticabal - Brazil

^[2] Sao Paulo State Univ, Lab Reprod Physiol, Sch Vet Med, UNESP, Aracatuba - Brazil

^[3] Sao Paulo State Univ, Dept Pharmacol, Inst Biosci, UNESP, Botucatu, SP - Brazil

^[4] Sao Paulo State Univ, Dept Biol Sci, Sch Sci Humanities & Languages, UNESP, Assis - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTION IN DOMESTIC ANIMALS; v. 54, n. 4, p. 666-677, APR 2019.
• Citações Web of Science: 1

Resumo

Contents The epidermal growth factor receptor (EGFR) pathway is directly involved in oocyte meiotic resumption induced by a gonadotropic stimulus. Here, we used an EGFR inhibitor (AG1478) to inhibit spontaneous meiosis resumption in bovine oocytes (EGFR- group) during 8 hr prematuration and assessed the competence of such oocytes for embryonic development, apoptosis and gene expression in comparison with Control group which was not prematured. Data are presented as mean +/- SEM. Blastocysts rate on day 7 (40.81%, averaged) and hatching rate on day 9 (77.35%, averaged) were unaffected by treatment (p > 0.05). Similarly, treatment did not affect (p > 0.05) the total cell number on day 7 (119.05, averaged) and on day 9 (189.5, averaged). Apoptosis was reduced (p < 0.05) in EGFR- group day 7-embryos compared to Control group (3.7% +/- 1.0 vs. 5.2% +/- 0.8). Abundance of several transcripts was upregulated (p < 0.05) in EGFR- group, including genes related to embryo development and quality (NANOG and RPLP0), epigenetic regulation (H2AFZ), apoptosis (BID) and stress response (GPX4 and HIF1A). Taken together, the results presented here demonstrated a reduction in the apoptosis index and upregulation of NANOG, H2AFZ and RPLP0 mRNA levels, which are related to embryonic development. Our data suggest that temporary meiosis blockage with EGFR inhibitor during prematuration culture of bovine oocytes may be an interesting strategy to improve embryo quality. (AU)

• Processo FAPESP: 12/50533-2 - GIFT: melhoramento genômico de características relacionadas com a fertilização em gado bovino dinamarquês e brasileiro
• Beneficiário: Marcelo Fábio Gouveia Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/06733-5 - Avaliação das características seminais em tourinhos da raça Nelore classificados para consumo alimentar residual (CAR) e suplementados com fonte de ácidos graxos poliinsaturados em regime de pastagem
• Beneficiário: Gisele Zoccal Mingoti
• Modalidade de apoio: Auxílio à Pesquisa - Regular