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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Differences in embryonic gene expression and quality indicate the benefit of epidermal growth factor receptor inhibitor during prematuration to improve competence in bovine oocytes

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Author(s):
Dall'Acqua, Priscila Chediek [1, 2] ; Nunes, Giovana Barros [1, 2] ; da Silva, Cintia Rodrigues [2] ; Fontes, Patricia Kubo [3] ; Gouveia Nogueira, Marcelo Fabio [3, 4] ; Lopes, Flavia Lombardi [2] ; Marinho, Marcia [2] ; Mingoti, Gisele Zoccal [1, 2]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ, UNESP, Dept Prevent Med & Anim Reprod, Sch Agr & Vet Sci, Jaboticabal - Brazil
[2] Sao Paulo State Univ, Lab Reprod Physiol, Sch Vet Med, UNESP, Aracatuba - Brazil
[3] Sao Paulo State Univ, Dept Pharmacol, Inst Biosci, UNESP, Botucatu, SP - Brazil
[4] Sao Paulo State Univ, Dept Biol Sci, Sch Sci Humanities & Languages, UNESP, Assis - Brazil
Total Affiliations: 4
Document type: Journal article
Source: REPRODUCTION IN DOMESTIC ANIMALS; v. 54, n. 4, p. 666-677, APR 2019.
Web of Science Citations: 0
Abstract

Contents The epidermal growth factor receptor (EGFR) pathway is directly involved in oocyte meiotic resumption induced by a gonadotropic stimulus. Here, we used an EGFR inhibitor (AG1478) to inhibit spontaneous meiosis resumption in bovine oocytes (EGFR- group) during 8 hr prematuration and assessed the competence of such oocytes for embryonic development, apoptosis and gene expression in comparison with Control group which was not prematured. Data are presented as mean +/- SEM. Blastocysts rate on day 7 (40.81%, averaged) and hatching rate on day 9 (77.35%, averaged) were unaffected by treatment (p > 0.05). Similarly, treatment did not affect (p > 0.05) the total cell number on day 7 (119.05, averaged) and on day 9 (189.5, averaged). Apoptosis was reduced (p < 0.05) in EGFR- group day 7-embryos compared to Control group (3.7% +/- 1.0 vs. 5.2% +/- 0.8). Abundance of several transcripts was upregulated (p < 0.05) in EGFR- group, including genes related to embryo development and quality (NANOG and RPLP0), epigenetic regulation (H2AFZ), apoptosis (BID) and stress response (GPX4 and HIF1A). Taken together, the results presented here demonstrated a reduction in the apoptosis index and upregulation of NANOG, H2AFZ and RPLP0 mRNA levels, which are related to embryonic development. Our data suggest that temporary meiosis blockage with EGFR inhibitor during prematuration culture of bovine oocytes may be an interesting strategy to improve embryo quality. (AU)

FAPESP's process: 15/06733-5 - Evaluation of seminal characteristics in Nellore bulls selected for residual feed intake and supplemented with polyunsaturated fatty acids in pasture conditions
Grantee:Gisele Zoccal Mingoti
Support type: Regular Research Grants
FAPESP's process: 12/50533-2 - GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle
Grantee:Marcelo Fábio Gouveia Nogueira
Support type: Research Projects - Thematic Grants