Characterization of novel hydrocarbon-degrading Gordonia paraffinivorans and Gordonia sihwensis strains isolated from composting

Autor(es):Mostrar menos -

Silva, Natalia Maria ^[1] ; Silva Araujo de Oliveira, Aline Marcia ^[1] ; Pegorin, Stefania ^[2] ; Giusti, Camila Escandura ^[1] ; Ferrari, Vitor Batista ^[3] ; Barbosa, Deibs ^[4] ; Martins, Layla Farage ^[4] ; Morais, Carlos ^[4] ; Setubal, Joao Carlos ^[4] ; Vasconcellos, Suzan Pantaroto ^[3] ; da Silva, Aline Maria ^[4] ; Franco de Oliveira, Julio Cezar ^[2] ; Pascon, Renata Castiglioni ^[2] ; Viana-Niero, Cristina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Ciencias Biol, Diadema - Brazil

^[3] Univ Fed Sao Paulo, Dept Ciencias Farmaceut, Diadema - Brazil

^[4] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 14, n. 4 APR 18 2019.
• Citações Web of Science: 0

Resumo

Hydrocarbons are important environmental pollutants, and the isolation and characterization of new microorganisms with the ability to degrade these compounds are important for effective biodegradation. In this work we isolated and characterized several bacterial isolates from compost, a substrate rich in microbial diversity. The isolates were obtained from selective culture medium containing n-hexadecane, aiming to recover alkane-degraders. Six isolates identified as Gordonia by MALDI-TOF and 16S rRNA sequencing had the ability to degrade n-hexadecane in three days. Two isolates were selected for genomic and functional characterization, Gordonia paraffinivorans (MTZ052) and Gordonia sihwensis (MTZ096). The CG-MS results showed distinct n-hexadecane degradation rates for MTZ052 and MTZ096 (86% and 100% respectively). The genome sequence showed that MTZ052 encodes only one alkane degrading gene cluster, the CYP153 system, while MTZ096 harbors both the Alkane Hydroxylase (AH) and the CYP153 systems. qPCR showed that both gene clusters are induced by the presence of n-hexadecane in the growth medium, suggesting that G. paraffinivorans and G. sihwensis use these systems for degradation. Altogether, our results indicate that these Gordonia isolates have a good potential for biotransformation of hydrocarbons. (AU)

• Processo FAPESP: 11/50870-6 - Estudos da diversidade microbiana no Parque Zoológico do Estado de São Paulo
• Beneficiário: João Carlos Setubal
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 16/23639-5 - Utilização de testes fenotípicos e moleculares para identificação de bactérias com capacidade de biodegradar hidrocarbonetos
• Beneficiário: Camila Escandura Giusti
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica