Multiplex-PCR for diagnosis of bacterial meningitis

Autor(es)

Albuquerque, Renata Chaves ^[1] ; Ramos Moreno, Ana Carolina ^{[1, 2]} ; dos Santos, Silvia Regina ^[3] ; Betta Ragazzi, Selma Lopes ^[3] ; Martinez, Marina Baquerizo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Clin Chem & Toxicol, Fac Ciencias Farmaceut, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Microbiol, Vaccine Dev Lab, Biomed Sci Inst, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Hosp Univ, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 50, n. 2, p. 435-443, APR 2019.
• Citações Web of Science: 0

Resumo

Considering the great lethality and sequels caused by meningitis, rapid diagnosis and prompt treatment initiation have a great impact on patient outcome. Here, we developed a multiplex-PCR for simultaneous detection of the four most prevalent bacterial pathogens directly in CSF samples. The multiplex-PCR was designed to detect the following genes: fbsA (Streptococcus agalactiae), lytA (Streptococcus pneumoniae), crtA (Neisseria meningitidis), p6 (Haemophilus influenzae), and 16S rRNA (any bacterial agent). The multiplex-PCR showed a DNA detection limit of 1pg/L. Among 447 CSF samples tested, 40 were multiplex-PCR positive, in which 27 and 13 had positive and negative bacterial culture, respectively. Our multiplex-PCR is fast, reliable, and easily implementable into a laboratory routine for bacterial meningitis confirmation, especially for patients who previously started antimicrobial therapy. Our molecular approach can substantially improve clinical diagnosis and epidemiological measures of meningitis disease burden. (AU)

• Processo FAPESP: 06/55141-4 - Biologia da escherichia coli enteroinvasora iv: efeitos da ativacao das celulas dendriticas intestinais na resposta inflamatoria.
• Beneficiário: Marina Baquerizo Martinez
• Modalidade de apoio: Auxílio à Pesquisa - Regular