| Full text | |
| Author(s): |
Albuquerque, Renata Chaves
[1]
;
Ramos Moreno, Ana Carolina
[1, 2]
;
dos Santos, Silvia Regina
[3]
;
Betta Ragazzi, Selma Lopes
[3]
;
Martinez, Marina Baquerizo
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Dept Clin Chem & Toxicol, Fac Ciencias Farmaceut, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Microbiol, Vaccine Dev Lab, Biomed Sci Inst, Sao Paulo - Brazil
[3] Univ Sao Paulo, Hosp Univ, Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Brazilian Journal of Microbiology; v. 50, n. 2, p. 435-443, APR 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Considering the great lethality and sequels caused by meningitis, rapid diagnosis and prompt treatment initiation have a great impact on patient outcome. Here, we developed a multiplex-PCR for simultaneous detection of the four most prevalent bacterial pathogens directly in CSF samples. The multiplex-PCR was designed to detect the following genes: fbsA (Streptococcus agalactiae), lytA (Streptococcus pneumoniae), crtA (Neisseria meningitidis), p6 (Haemophilus influenzae), and 16S rRNA (any bacterial agent). The multiplex-PCR showed a DNA detection limit of 1pg/L. Among 447 CSF samples tested, 40 were multiplex-PCR positive, in which 27 and 13 had positive and negative bacterial culture, respectively. Our multiplex-PCR is fast, reliable, and easily implementable into a laboratory routine for bacterial meningitis confirmation, especially for patients who previously started antimicrobial therapy. Our molecular approach can substantially improve clinical diagnosis and epidemiological measures of meningitis disease burden. (AU) | |