Participation of integrin beta 3 in osteoblast differentiation induced by titanium with nano or microtopography

Autor(es)

Lopes, Helena B. ^[1] ; Freitas, Gileade P. ^[1] ; Elias, Carlos N. ^[2] ; Tye, Coralee ^[3] ; Stein, Janet L. ^[3] ; Stein, Gary S. ^[3] ; Lian, Jane B. ^[3] ; Rosa, Adalberto L. ^[1] ; Beloti, Marcio M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Cell Culture Lab, Sch Dent Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[2] Inst Mil Engn, Biomat Lab, Rio De Janeiro, RJ - Brazil

^[3] Univ Vermont, Dept Biochem, Canc Ctr, Burlington, VT 05405 - USA

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biomedical Materials Research Part A; v. 107, n. 6, p. 1303-1313, JUN 2019.
• Citações Web of Science: 2

Resumo

The major role of integrins is to mediate cell adhesion but some of them are involved in the osteoblasts-titanium (Ti) interactions. In this study, we investigated the participation of integrins in osteoblast differentiation induced by Ti with nanotopography (Ti-Nano) and with microtopography (Ti-Micro). By using a PCR array, we observed that, compared with Ti-Micro, Ti-Nano upregulated the expression of five integrins in mesenchymal stem cells, including integrin beta 3, which increases osteoblast differentiation. Silencing integrin beta 3, using CRISPR-Cas9, in MC3T3-E1 cells significantly reduced the osteoblast differentiation induced by Ti-Nano in contrast to the effect on T-Micro. Concomitantly, integrin beta 3 silencing downregulated the expression of integrin alpha v, the parent chain that combines with other integrins and several components of the Wnt/beta-catenin and BMP/Smad signaling pathways, all involved in osteoblast differentiation, only in cells cultured on Ti-Nano. Taken together, our results showed the key role of integrin beta 3 in the osteogenic potential of Ti-Nano but not of Ti-Micro. Additionally, we propose a novel mechanism to explain the higher osteoblast differentiation induced by Ti-Nano that involves an intricate regulatory network triggered by integrin beta 3 upregulation, which activates the Wnt and BMP signal transductions. (c) 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1303-1313, 2019. (AU)

• Processo FAPESP: 13/05181-3 - Participação de integrinas na diferenciação osteoblástica de células-tronco mesenquimais induzida por superfícies de titânio com micro e nanotopografia
• Beneficiário: Márcio Mateus Beloti
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/21116-5 - Uso do CRISPR-Cas9 na Investigação do Papel das Integrinas na Diferenciação Osteoblastica de Células-Tronco Mesenquimais Induzidas por Superfícies de Titânio
• Beneficiário: Helena Bacha Lopes
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 14/08443-1 - Participação de integrinas na diferenciação osteoblástica de células-tronco mesenquimais induzida por superfícies de titânio com micro e nanotopografia
• Beneficiário: Helena Bacha Lopes
• Modalidade de apoio: Bolsas no Brasil - Doutorado