Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Participation of integrin beta 3 in osteoblast differentiation induced by titanium with nano or microtopography

Full text
Author(s):
Lopes, Helena B. [1] ; Freitas, Gileade P. [1] ; Elias, Carlos N. [2] ; Tye, Coralee [3] ; Stein, Janet L. [3] ; Stein, Gary S. [3] ; Lian, Jane B. [3] ; Rosa, Adalberto L. [1] ; Beloti, Marcio M. [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Cell Culture Lab, Sch Dent Ribeirao Preto, Ribeirao Preto, SP - Brazil
[2] Inst Mil Engn, Biomat Lab, Rio De Janeiro, RJ - Brazil
[3] Univ Vermont, Dept Biochem, Canc Ctr, Burlington, VT 05405 - USA
Total Affiliations: 3
Document type: Journal article
Source: Journal of Biomedical Materials Research Part A; v. 107, n. 6, p. 1303-1313, JUN 2019.
Web of Science Citations: 2
Abstract

The major role of integrins is to mediate cell adhesion but some of them are involved in the osteoblasts-titanium (Ti) interactions. In this study, we investigated the participation of integrins in osteoblast differentiation induced by Ti with nanotopography (Ti-Nano) and with microtopography (Ti-Micro). By using a PCR array, we observed that, compared with Ti-Micro, Ti-Nano upregulated the expression of five integrins in mesenchymal stem cells, including integrin beta 3, which increases osteoblast differentiation. Silencing integrin beta 3, using CRISPR-Cas9, in MC3T3-E1 cells significantly reduced the osteoblast differentiation induced by Ti-Nano in contrast to the effect on T-Micro. Concomitantly, integrin beta 3 silencing downregulated the expression of integrin alpha v, the parent chain that combines with other integrins and several components of the Wnt/beta-catenin and BMP/Smad signaling pathways, all involved in osteoblast differentiation, only in cells cultured on Ti-Nano. Taken together, our results showed the key role of integrin beta 3 in the osteogenic potential of Ti-Nano but not of Ti-Micro. Additionally, we propose a novel mechanism to explain the higher osteoblast differentiation induced by Ti-Nano that involves an intricate regulatory network triggered by integrin beta 3 upregulation, which activates the Wnt and BMP signal transductions. (c) 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1303-1313, 2019. (AU)

FAPESP's process: 14/08443-1 - Role of integrins on the osteoblast differentiation of mesenchymal stem cells induced by titanium surfaces with micro and nanotopography
Grantee:Helena Bacha Lopes
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/05181-3 - Role of integrins on the osteoblast differentiation of mesenchymal stem cells induced by titanium surfaces with micro and nanotopography
Grantee:Márcio Mateus Beloti
Support type: Regular Research Grants
FAPESP's process: 16/21116-5 - Use of CRISPR-Cas9 to Investigate the Role of Integrins on Osteoblast Differentiation of Mesenchymal Stem Cells Induced by Titanium Surfaces
Grantee:Helena Bacha Lopes
Support type: Scholarships abroad - Research Internship - Doctorate