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Effect of titanium nanotopography on osteoblastic differentiation and alpha1 beta1 integrin expression of mesenchymal stem cells derived from bone marrow and cells derived from rat calvaria

Grant number: 15/00128-2
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2015
Effective date (End): April 30, 2016
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Márcio Mateus Beloti
Grantee:Pedro Flório
Home Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Studies from our research group have shown that the nanotopography of titanium (Ti) surface, produced by H2SO4/H2O2 treatment, favors osteoblastic differentiation through microRNA-SMAD-BMP-2 circuit and a1b1 integrin signaling pathway. These results generated a new project supported by FAPESP to deeply investigate the participation of integrins in cell/nanotopography interactions, using mesenchymal stem cells (MSCs). However, previous results suggest that the osteogenic potential of nanotopography is more evident in osteoblastic cells derived from rat calvaria, despite the response of these cell cultures to the nanotpography was not compared, yet. Therefore, to obtain relevant information regarding the most suitable cell culture model to the development of the main project, focused on participation of integrins in the osteogenic potential of nanotopography, the aim of this study is to evaluate and compare the effect of nanotopography on osteoblastic differentiation and a1 b1 integrin expression of MSCs derived from rat bone marrow and cells derived from rat calvaria. In this way, cells from both sources will be cultured on discs of Ti with nanotopography to evaluate: (1) gene expression of osteoblast markers, Runx2, Osterix, alkaline phosphatase (ALP), osteocalcin (OC), osteopontin (OPN) and bone sialoprotein (BSP), and a1 and b1 integrins by real-time PCR, at day 10 and (2) extracellular matrix mineralization with alizarin red stain, at day 17. The data will be submitted to the Mann-Whitney test to compare both cultures grown on Ti with nanotopography.