Evaluation of neuroglobin and cytoglobin expression in adult rats exposed to silver nanoparticles during prepubescence

Autor(es)

da Conceicao, Rodrigo Rodrigues ^[1] ; de Souza, Janaina Sena ^[1] ; de Oliveira, Kelen Carneiro ^[1] ; Romano, Renata Marino ^[2] ; de Barros Maciel, Rui Monteiro ^[1] ; Dias-da-Silva, Magnus Regios ^[1] ; Romano, Marco Aurelio ^[2] ; Chiamolera, Maria Izabel ^[1] ; Giannocco, Gisele ^{[3, 1]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Dept Med, Disciplina Endocrinol Clin, Lab Endocrinol Mol & Translac, Rua Pedro Toledo 669, BR-04039032 Sao Paulo, SP - Brazil

^[2] State Univ Ctr Oeste, Dept Pharm, Lab Reprod Toxicol, Rua Simeao Camargo Varela Sa 03, BR-85040080 Guarapuava, Parana - Brazil

^[3] Univ Fed Sao Paulo, Dept Ciencias Biol, Diadema, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: METABOLIC BRAIN DISEASE; v. 34, n. 3, p. 705-713, JUN 2019.
• Citações Web of Science: 0

Resumo

Silver nanoparticles (AgNPs) are clusters of silver atoms with diameters that range from 1 to 100nm. Due to the various shapes and large surface areas, AgNPs have been employed in the food and textile industries and medical fields. Therefore, because of the widespread use of these compounds, the aim of this study was to evaluate the effect of AgNP exposure on the gene and protein expression levels of Neuroglobin (Ngb) and Cytoglobin (Cygb), in the rat cortex, hippocampus and cerebellum. Post-natal day (PND) 21 male Wistar rats were randomly divided into three groups. One group received 15g/kg body weight of AgNP by gavage another group received 30g/kg and the control group that received saline, from PND23 to PND58. On PND102 the animals were euthanized and the cortex, hippocampus and cerebellum were isolated and evaluated for gene and protein expression levels of Nbg and Cygb. The results demonstrated that the 30g/kg AgNP group displayed increased gene and protein expression of Cygb in the cortex. In the Hippocampus, AgNP exposure did not modulate gene or protein expression levels of Ngb and Cygb. In cerebellum the Ngb gene and protein expression was increased with both doses of AgNP. AgNP exposure during prepubescence can modulate the gene and protein expression levels of Ngb and Cygb in adulthood. Furthermore, the observed modulation was specific to the cerebellum, and cortex, and was dose dependent. (AU)

• Processo FAPESP: 13/26851-7 - Estudo in vivo do efeito dos desreguladores endócrinos glifosato e bisfenol-A sobre o eixo hipotálamo-hipófise-tiroide
• Beneficiário: Maria Izabel Chiamolera
• Modalidade de apoio: Auxílio à Pesquisa - Regular