Exploring Collagen Parameters in Pure Special Types of Invasive Breast Cancer

Autor(es):Mostrar menos -

Natal, Rodrigo de Andrade ^[1] ; Paiva, Geisilene R. ^[2] ; Pelegati, Vitor B. ^[3] ; Marenco, Ludwing ^[3] ; Alvarenga, Cesar A. ^[4] ; Vargas, Renato F. ^[2] ; Derchain, Sophie F. ^[5] ; Sarian, Luis O. ^[5] ; Franchet, Camille ^[6] ; Cesar, Carlos L. ^{[3, 7]} ; Schmitt, Fernando C. ^{[8, 9]} ; Weigelt, Britta ^[10] ; Vassallo, Jose ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, CIPED Fac Med Sci, Lab Investigative & Mol Pathol, Rua Tessalia Vieira de Camargo 126, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, LAPE Fac Med Sci, Lab Specialized Pathol, Rua Tessalia Vieira de Camargo 126, BR-13083970 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Inst Phys Gleb Wataghin, Dept Quantum Elect, Rua Sergio Buarque de Holanda 777, BR-13083859 Campinas, SP - Brazil

^[4] Inst Patol Campinas, Av Andrade Neves 1801, BR-13070000 Campinas, SP - Brazil

^[5] Univ Estadual Campinas, Fac Med Sci, Dept Obstet & Gynecol, Rua Tessalia Vieira de Camargo 126, BR-13083970 Campinas, SP - Brazil

^[6] Univ Canc Inst, Dept Pathol, Ave Irene Joliot Curie 1, F-31059 Toulousse - France

^[7] Fed Univ Ceara UFC, Dept Phys, Campus Pici, Bloco 922, BR-60455760 Fortaleza, Ceara - Brazil

^[8] Univ Porto, Inst Mol Pathol & Immunol, IPATIMUP, Rua Dr Roberto Frias S-N, BR-60455760 Fortaleza, Ceara - Brazil

^[9] Natl Sante Lab, Dept Med, L-3555 Dudelange - Luxembourg

^[10] Mem Sloan Kettering Canc Ctr, Dept Pathol, New York, NY 10065 - USA

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, MAY 22 2019.
• Citações Web of Science: 1

Resumo

One of the promising tools to evaluate collagen in the extracellular matrix is the second-harmonic generation microscopy (SHG). This approach may shed light on the biological behavior of cancers and their taxonomy, but has not yet been applied to characterize collagen fibers in cases diagnosed as invasive breast carcinoma (BC) of histological special types (IBC-ST). Tissue sections from 99 patients with IBC-ST and 21 of invasive breast carcinoma of no special type (IBC-NST) were submitted to evaluation of collagen parameters by SHG. Tissue microarray was performed to evaluate immunohistochemical-based molecular subtype. In intratumoral areas, fSHG and bSHG (forward-SHG and backward-SHG) collagen parameters achieved their lowest values in mucinous, papillary and medullary carcinomas, whereas the highest values were found in classic invasive lobular and tubular carcinomas. Unsupervised hierarchical cluster analysis and minimal spanning tree using intratumoral collagen parameters allowed the identification of three main groups of breast cancer: group A (classic invasive lobular and tubular carcinomas); group B (IBC-NST, metaplastic, invasive apocrine and micropapillary carcinomas); and group C (medullary, mucinous and papillary carcinomas). Our findings provide further characterization of the tumor microenvironment of IBC-ST. This understanding may add information to build more consistent tumor categorization and to refine prognostication. (AU)

• Processo FAPESP: 11/51959-0 - Biologia das doenças neoplásicas da medula óssea
• Beneficiário: Sara Teresinha Olalla Saad
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 08/57906-3 - Instituto Nacional de Fotônica Aplicada à Biologia Celular - INFABIC
• Beneficiário: Hernandes Faustino de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Temático