DNA methylation screening after roux-en Y gastric bypass reveals the epigenetic signature stems from genes related to the surgery per se

Autor(es)

Nicoletti, C. F. ^[1] ; Pinhel, M. A. S. ^[1] ; Diaz-Lagares, A. ^{[2, 3]} ; Casanueva, F. F. ^{[4, 5, 6]} ; Jacome, A. ^[7] ; Pinhanelli, V. C. ^[1] ; de Oliveira, B. A. P. ^[1] ; Crujeiras, A. B. ^{[4, 5, 6]} ; Nonino, C. B. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Internal Med, Lab Nutrigen Studies, Sao Paulo - Brazil

^[2] Ctr Invest Biomed Red Canc CIBERONC, Madrid - Spain

^[3] Univ Clin Hosp Santiago CHUS SERGAS, Hlth Res Inst Santiago IDIS, Canc Epigen Translat Med Oncol Oncomet, Santiago De Compostela - Spain

^[4] Univ Clin Hosp Santiago CHUS SERGAS, Hlth Res Inst Santiago IDIS, Epigen Endocrinol & Nutr, Santiago De Compostela - Spain

^[5] Santiago de Compostela Univ USC, Santiago De Compostela - Spain

^[6] CIBER Fisiopatol Obesidad & Nutr CIBERobn, Madrid - Spain

^[7] Univ A Coruna, CITIC, MODES Grp, Dept Math, Fac Sci, La Coruna - Spain

• Tipo de documento: Artigo Científico
• Fonte: BMC MEDICAL GENOMICS; v. 12, MAY 27 2019.
• Citações Web of Science: 0

Resumo

Background/objectivesObesity has been associated with gene methylation regulation. Recent studies have shown that epigenetic signature plays a role in metabolic homeostasis after Roux-en Y gastric bypass (RYGB). To conduct a genome-wide epigenetic analysis in peripheral blood to investigate whether epigenetic changes following RYGB stem from weight loss or the surgical procedure per se.Subjects/methodsBy means of the Infinium Human Methylation 450 BeadChip array, global methylation was analyzed in blood of 24 severely obese women before and 6 months after RYGB and in 24 normal-weight women (controls).ResultsIn blood cells, nine DMCpG sites showed low methylation levels before surgery, methylation levels increased after RYGB and neared the levels measured in the controls. Additionally, 44 CpG sites associated with the Wnt and p53 signaling pathways were always differently methylated in the severely obese patients as compared to the controls and were not influenced by RYGB. Finally, 1638 CpG sites related to inflammation, angiogenesis, and apoptosis presented distinct methylation in the post-surgery patients as compared to the controls.ConclusionBariatric surgery per se acts on CpGs related to inflammation, angiogenesis, and endothelin-signaling. However, the gene cluster associated with obesity remains unchanged, suggesting that weight loss 6 months after RYGB surgery cannot promote this effect. (AU)

• Processo FAPESP: 17/07220-7 - Aplicação de ferramentas de bioinformática para análise do perfil de metilação do DNA antes e após a cirurgia bariátrica
• Beneficiário: Carolina Nicoletti Ferreira Fino
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 16/05638-1 - Padrão de metilação global do DNA antes e após tratamentos para perda de peso
• Beneficiário: Carla Barbosa Nonino
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/18669-0 - Avaliação do padrão de metilação do DNA antes e após derivação gástrica em Y-de-Roux
• Beneficiário: Carolina Nicoletti Ferreira Fino
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado