Pradimicin-IRD from Amycolatopsis sp. IRD-009 and its antimicrobial and cytotoxic activities

Autor(es)

Bauermeister, Anelize ^[1] ; Calil, Felipe A. ^[1] ; Pinto, Francisco das C. L. ^[2] ; Medeiros, Talita C. T. ^[1] ; Almeida, Larissa C. ^[3] ; Silva, Leonardo J. ^[4] ; de Melo, Itamar S. ^[5] ; Zucchi, Tiago D. ^[6] ; Costa-Lotufo, Leticia V. ^[3] ; Moraes, Luiz A. B. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ribeirao Preto - Brazil

^[2] Univ Fed Ceara, Dept Quim Organ & Inorgan, Fortaleza, Ceara - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Farmacol, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Coll Agr Luiz de Queiroz, Piracicaba - Brazil

^[5] Natl Brazilian Res Corp EMBRAPA ENVIRONM, Lab Environm Microbiol, Jaguariuna - Brazil

^[6] AGRIVALLE, Dept Pesquisa & Desenvolvimento, Salto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NATURAL PRODUCT RESEARCH; v. 33, n. 12, p. 1713-1720, JUN 18 2019.
• Citações Web of Science: 2

Resumo

A new polycyclic antibiotic, pradimicin-IRD, was isolated from actinobacteria Amycolatopsis sp. IRD-009 recovered from soil of Brazilian rainforest undergoing restoration area. This molecule is the major compound produced in solid culture media. The new compound was detected by a focused method of precursor ion (high-performance liquid chromatography coupled to tandem mass spectrometer) developed previously to identify unusual aminoglycosyl sugar moieties. The compound was isolated and its structure was, therefore, elucidated by high-resolution mass spectrometry, and 1D and 2D nuclear magnetic resonance experiments. Pradimicin-IRD displayed potential antimicrobial activity against Streptococcus agalactiae (MIC 3.1 mu g/mL), Pseudomonas aeruginosa (MIC 3.1 mu g/mL) and Staphylococcus aureus (MIC 3.1 mu g/mL), and also cytotoxicity against tumour and non-tumour cell lines with IC50 values ranging from 0.8 mu M in HCT-116 colon carcinoma cells to 2.7 mu M in MM 200 melanoma cells. Particularly, these biological properties are described for the first time for this chemical class. {[}GRAPHICS] . (AU)

• Processo FAPESP: 13/50729-7 - DESI-HP-TLC-Bioautografia: uma nova estratégia para identificação rápida de metabólitos secundários anti-mastítico, produzido por actinobactérias
• Beneficiário: Luiz Alberto Beraldo de Moraes
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 17/17648-4 - Abordagem integrada na prospecção sustentável de produtos naturais marinhos: da diversidade a substâncias anticâncer
• Beneficiário: Anelize Bauermeister
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado