| Full text | |
| Author(s): |
Bauermeister, Anelize
[1]
;
Calil, Felipe A.
[1]
;
Pinto, Francisco das C. L.
[2]
;
Medeiros, Talita C. T.
[1]
;
Almeida, Larissa C.
[3]
;
Silva, Leonardo J.
[4]
;
de Melo, Itamar S.
[5]
;
Zucchi, Tiago D.
[6]
;
Costa-Lotufo, Leticia V.
[3]
;
Moraes, Luiz A. B.
[1]
Total Authors: 10
|
| Affiliation: | [1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ribeirao Preto - Brazil
[2] Univ Fed Ceara, Dept Quim Organ & Inorgan, Fortaleza, Ceara - Brazil
[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Farmacol, Sao Paulo - Brazil
[4] Univ Sao Paulo, Coll Agr Luiz de Queiroz, Piracicaba - Brazil
[5] Natl Brazilian Res Corp EMBRAPA ENVIRONM, Lab Environm Microbiol, Jaguariuna - Brazil
[6] AGRIVALLE, Dept Pesquisa & Desenvolvimento, Salto - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | NATURAL PRODUCT RESEARCH; v. 33, n. 12, p. 1713-1720, JUN 18 2019. |
| Web of Science Citations: | 2 |
| Abstract | |
A new polycyclic antibiotic, pradimicin-IRD, was isolated from actinobacteria Amycolatopsis sp. IRD-009 recovered from soil of Brazilian rainforest undergoing restoration area. This molecule is the major compound produced in solid culture media. The new compound was detected by a focused method of precursor ion (high-performance liquid chromatography coupled to tandem mass spectrometer) developed previously to identify unusual aminoglycosyl sugar moieties. The compound was isolated and its structure was, therefore, elucidated by high-resolution mass spectrometry, and 1D and 2D nuclear magnetic resonance experiments. Pradimicin-IRD displayed potential antimicrobial activity against Streptococcus agalactiae (MIC 3.1 mu g/mL), Pseudomonas aeruginosa (MIC 3.1 mu g/mL) and Staphylococcus aureus (MIC 3.1 mu g/mL), and also cytotoxicity against tumour and non-tumour cell lines with IC50 values ranging from 0.8 mu M in HCT-116 colon carcinoma cells to 2.7 mu M in MM 200 melanoma cells. Particularly, these biological properties are described for the first time for this chemical class. {[}GRAPHICS] . (AU) | |
| FAPESP's process: | 13/50729-7 - DESI-HPTLC-Bioautography: New strategies to rapid Identification of anti-mastotos bioactive metabolites from actinomycetes. |
| Grantee: | Luiz Alberto Beraldo de Moraes |
| Support Opportunities: | BIOTA-FAPESP Program - Regular Research Grants |
| FAPESP's process: | 17/17648-4 - Integrative approach on the sustainable prospection of marine natural products: from diversity to anticancer compounds |
| Grantee: | Anelize Bauermeister |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |