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Anti-Candida, antiproliferative and cytotoxic activities evaluation of essential oil and fractions from Coriandrum sativum L.

Grant number: 11/15984-0
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): May 01, 2012
Effective date (End): February 28, 2014
Field of knowledge:Health Sciences - Dentistry - Social and Preventive Dentistry
Principal Investigator:Pedro Luiz Rosalen
Grantee:Irlan de Almeida Freires
Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil


Oral cavity is colonized by a mixed microbiota that may overcome host defense mechanisms and act opportunistically, leading to numerous clinical manifestations. Oral candidiasis, superficial fungal infection caused by Candida yeasts, has become more and more prevalent. That might be due to the indiscriminate prophylactic use of antifungal agents. Fungal resistance to drugs allied to the limited availability of therapeutic class of antifungals drive researches to the discovery of new agents from other sources. Literature highlights interest in natural products as a source of new bioactive molecules to control oral affections. Among those products are the essential oils (EO) and their secondary products which present antimicrobial properties. The need to generate and aggregate new information on in vitro antimicrobial activity of EO has motivated the conduction of this study, which involves a species from the Germplasm Bank of the "Collection of Medicinal and Aromatic Plants" - CPMA / UNICAMP (State University of Campinas). Among many species with promising antimicrobial activity, especially as antifungal agents, literature cites Coriandrum sativum L., popularly known as coriander. The present investigation aims at continuing the studies on such aromatic species, which had already been supported by FAPESP. In that context, it is aimed to identify the in vitro antimicrobial activity of the EO and fractions from C. sativum upon Candida yeasts, as well as to identify the classes and bioactive chemical substances present in the EO and in its fractions showing the best activity. Moreover, the EO and fractions will undergo antiproliferative and cytotoxic analyses through cell culture. Finally, it will be verified the ability of C. sativum and its active fractions in inhibiting the proteolytic activity of proteinases and phospholipases produced by Candida albicans. The EO and fractions of C. sativum, extracted of plants from the Germplasm Bank of the CPMA of Multidisciplinary Center for Chemical, Biological and Agricultural Researches (CPQBA)/ UNICAMP, will be evaluated with regards to their antimicrobial activity upon oral Candida spp. yeasts (species: C. albicans - CBS-562, C. dubliniensis - CBS-7987, C. krusei - CBS-573, C. tropicalis - CBS-94 and C. parapsilosis - CBS-604) by using the following methodologies: determination of Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) and inhibition of the proteolytic activity of proteases; it will be also verified the antiproliferative activity on human skin fibroblasts ATCC-CRL 2522TM and tumor cell lineages. Classes of the bioactive chemical substances present in the EO and fractions will be identified by RP-HPLC and GC-MS. It is expected to determine the antimicrobial potential of the EO under study upon oral yeasts and to provide additional information to the Germplasm Bank of the CPQBA/ UNICAMP and to literature so contributing to future studies on action mechanism, toxicology and chemical isolation of compounds that might be promising in the production of dental drug formulations. (AU)

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Scientific publications (8)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FREIRES, IRLAN ALMEIDA; DE ALENCAR, SEVERINO MATIAS; ROSALEN, PEDRO LUIZ. A pharmacological perspective on the use of Brazilian Red Propolis and its isolated compounds against human diseases. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, v. 110, p. 267-279, . (11/15984-0, 13/25080-7)
FREIRES, IRLAN ALMEIDA; ROSALEN, PEDRO LUIZ. How Natural Product Research has Contributed to Oral Care Product Development? A Critical View. PHARMACEUTICAL RESEARCH, v. 33, n. 6, p. 1311-1317, . (11/15984-0, 13/25080-7)
ORLANDI SARDI, JANAINA DE CASSIA; FREIRES, IRLAN ALMEIDA; LAZARINI, JOSY GOLDONI; INFANTE, JULIANA; DE ALENCAR, SEVERINO MATIAS; ROSALEN, PEDRO LUIZ. Unexplored endemic fruit species from Brazil: Antibiofilm properties, insights into mode of action, and systemic toxicity of four Eugenia spp.. Microbial Pathogenesis, v. 105, p. 280-287, . (11/15984-0, 13/26251-0)
FREIRES, IRLAN ALMEIDA; DE CASSIA ORLANDI SARDI, JANAINA; DE CASTRO, RICARDO DIAS; ROSALEN, PEDRO LUIZ. Alternative Animal and Non-Animal Models for Drug Discovery and Development: Bonus or Burden?. PHARMACEUTICAL RESEARCH, v. 34, n. 4, p. 681-686, . (11/15984-0, 13/25080-7)
FREIRES, IRLAN ALMEIDA; BUENO-SILVA, BRUNO; DE CARVALHO GALVAO, LIVIA CAMARA; TEIXEIRA DUARTE, MARTA CRISTINA; SARTORATTO, ADILSON; FIGUEIRA, GLYN MARA; DE ALENCAR, SEVERINO MATIAS; ROSALEN, PEDRO LUIZ. The Effect of Essential Oils and Bioactive Fractions on Streptococcus mutans and Candida albicans Biofilms: A Confocal Analysis. Evidence-based Complementary and Alternative Medicine, . (12/01500-4, 11/15984-0, 09/12353-0, 11/14757-0)
FREIRES, IRLAN DE ALMEIDA; MURATA, RAMIRO MENDONCA; FURLETTI, VIVIAN FERNANDES; SARTORATTO, ADILSON; DE ALENCAR, SEVERINO MATIAS; FIGUEIRA, GLYN MARA; DE OLIVEIRA RODRIGUES, JANAINA APARECIDA; TEIXEIRA DUARTE, MARTA CRISTINA; ROSALEN, PEDRO LUIZ. Coriandrum sativum L. (Coriander) Essential Oil: Antifungal Activity and Mode of Action on Candida spp., and Molecular Targets Affected in Human Whole-Genome Expression. PLoS One, v. 9, n. 6, . (11/15984-0, 11/14757-0)
FREIRES, IRIAN ALMEIDA; SANTAELLA, GUSTAVO MACHADO; ORLANDI SARDI, JANAINA DE CASSIA; ROSALEN, PEDRO LUIZ. The alveolar bone protective effects of natural products: A systematic review. ARCHIVES OF ORAL BIOLOGY, v. 87, p. 196-203, . (11/15984-0, 13/25080-7)
FREIRES, IRLAN ALMEIDA; DENNY, CARINA; BENSO, BRUNA; DE ALENCAR, SEVERINO MATIAS; ROSALEN, PEDRO LUIZ. Antibacterial Activity of Essential Oils and Their Isolated Constituents against Cariogenic Bacteria: A Systematic Review. Molecules, v. 20, n. 4, p. 7329-7358, . (11/15984-0, 13/25080-7, 11/14757-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FREIRES, Irlan de Almeida. Atividade antifúngica e modo de ação do óleo essencial de Coriandrum sativum L. (coentro) sobre Candida spp. e alvos moleculares afetados na expressão do genoma humano. 2014. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.

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