Integrated chemical and biological study in the search for bioactive substances in plants* and development of analytical procedures. *(Amaranthaceae, Gesneriaceae, Myrtaceae e Annonaceae)

• Grant number: 15/03726-8
• Support type: Regular Research Grants
• Duration: July 01, 2015 - September 30, 2017
• Field of knowledge: Health Sciences - Pharmacy - Pharmacognosy
• Principal researcher: Marcos José Salvador
• Grantee: Marcos José Salvador
• Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

In this project is proposed the chemical study and evaluation of biological activities to search for bioactive compounds in plant species (mainly, Amaranthaceae, Gesneriaceae, Myrtaceae and Annonaceae) aiming integrated and continued studies within the chemical area of natural products with emphasis in the search for compounds (volatile and non-volatile) with a potential antimicrobial activity, antitumor or anti-inflammatory in the presence or absence of laser irradiation aiming to application in photodynamic therapy (PDT). To do so, the development of analytical procedures will be performed, including phytochemical study monitored by absorption analysis in the visible region of interest on PDT, assessing the photochemical efficiency, chromatographic profile, ESI-MS analysis and the bioassays. Proceed will be adequacy of biological testing methodology and procedures for assessing the biological effect photoinduced for natural products such as antimicrobial (against bacteria, fungi and protozoa), antiproliferative (against tumor cell lines and against non-tumor cells such as human keratinocyte and fibroblasts), and anti-inflammatory activities, so that the bioassay is likely to be applied to the separation methodology and characterization of bioactive chemical constituents present in the samples. Therefore, plant species will be collected and their extracts prepared, and then submitted to bioassays sequence, in order to select those with potential biological activity. The promising extracts in the bioactivity evaluation will be fractionated and for active metabolites purified their molecular structures will be determinated using spectroscopic methods of analysis (IR, UV-vis, NMR 1D and 2D and Mass spectrometry). In addition, the volatile oils from aromatic species could be obtained, which will be chemically analyzed using GC-MS analysis and subjected to the evaluation of the biological potential. Prospecting study will be performed for the research of bioactive agents accumulated in Brazilian plant species in order to contribute to sustainable use of biodiversity and obtaining new inputs and technologies for application in pharmaceuticals, cosmetics and dental, aimed at possible development of drugs and medicines. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA, CRISTHIAN SANTOS; ALVAREZ, CARLOS JOSE; PEREIRA CABRAL, MARCIA REGINA; SARRAGIOTTO, MARIA HELENA; SALVADOR, MARCOS JOSE; ALVES STEFANELLO, MARIA ELIDA. Three new diterpenoids from the leaves of Salvia lachnostachys. NATURAL PRODUCT RESEARCH, DEC 2021. (17/03598-5, 12/09101-1, 06/06079-4, 15/03726-8)

RAMIREZ-RUEDA, ROMAN Y.; SALVADOR, MARCOS J.. Phenotypic detection of quorum sensing inhibition inPseudomonas aeruginosapyoverdine and swarming by volatile organic products. FUTURE MICROBIOLOGY, v. 15, n. 12, p. 1147-1156, AUG 2020. (15/03726-8)

DE OLIVEIRA, MARIA A. C.; VEGIAN, MARIANA R.; BRIGHENTI, FERNANDA L.; SALVADOR, MARCOS J.; KOGA-ITO, CRISTIANE Y.. Antibiofilm effects of Thymus vulgaris and Hyptis spicigera essential oils on cariogenic bacteria. FUTURE MICROBIOLOGY, v. 16, n. 4, FEB 2021. (15/03726-8)

KASSUYA, ROBERTO MIKIO; DOS SANTOS, ELISANGELA; BOSSO, FERNANDO HENRIQUE; PEDROSO, TAISE FONSECA; MARINHO, JANE VASCONCELOS NEVES; SALVADOR, MARCOS JOSE; KASSUYA, CANDIDA APARECIDA LEITE; GASPAROTTO JUNIOR, ARQUIMEDES. Anti-inflammatory Properties of Ethanolic Extract and 2 `'-O-beta-d-Glucopyranosyl-vitexin Obtained from Alternanthera tenella Colla Whole Plant. Inflammation, v. 44, n. 4, p. 1540-1552, AUG 2021. (17/09781-6, 15/03726-8, 06/06079-4)

SERAIN, ALESSANDRA F.; MOROSI, LAVINIA; CERUTI, TOMMASO; MATTEO, CRISTINA; MERONI, MARINA; MINATEL, ELAINE; ZUCCHETTI, MASSIMO; SALVADOR, MARCOS J.. Betulinic acid and its spray dried microparticle formulation: In vitro PDT effect against ovarian carcinoma cell line and in vivo plasma and tumor disposition. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 224, NOV 2021. (14/26320-4, 15/03726-8, 17/19523-4, 16/06407-3)

SERAIN, A. F.; SILVERIO, S. E. B.; DE LOURENCO, C. C.; NUNES, V. K.; CORREA, W. R.; STEFANELLO, M. E. A.; SALVADOR, M. J.. Development of Sinningia magnifica (Otto & A. Dietr.) Wiehler (Gesneriaceae) tissue culture for in vitro production of quinones and bioactive molecules. INDUSTRIAL CROPS AND PRODUCTS, v. 159, JAN 2021. (15/03726-8, 17/19523-4, 14/26320-4, 16/06407-3)

VANESSA WINIEWSKI; ALESSANDRA F. SERAIN; EDUARDO L. DE SÁ; MARCOS J. SALVADOR; MARIA ÉLIDA A. STEFANELLO. CHEMICAL CONSTITUENTS OF Sinningia mauroana AND SCREENING OF ITS EXTRACTS FOR ANTIMICROBIAL, ANTIOXIDANT AND CYTOTOXIC ACTIVITIES. Química Nova, v. 43, n. 2, p. 181-187, Fev. 2020. (16/06407-3, 15/03726-8)

SILVA, ADSON S.; AMORIM, MAGALI S.; FONSECA, MARIANA M.; SALVADOR, MARCOS J.; DE SA, EDUARDO L.; STEFANELLO, MARIA ELIDA A.. A New Cytotoxic Naphthoquinone and Other Chemical Constituents of Sinningia reitzii. Journal of the Brazilian Chemical Society, v. 30, n. 10, SI, p. 2060-2065, OCT 2019. (15/03726-8, 17/03598-5)

MESQUITA, M.; SANTOS, E.; KASSUYA, C. A.; SALVADOR, M. J.. Chimarra tilde o, terere and mate-tea in legitimate technology modes of preparation and consume: A comparative study of chemical composition, antioxidant, anti-inflammatory and anti-anxiety properties of the mostly consumed beverages of Ilex paraguariensis St. Hil.. Journal of Ethnopharmacology, v. 279, OCT 28 2021. (17/03598-5, 06/06079-4, 12/09101-1, 15/03726-8)

TOMASIN, REBEKA; RHEDER FAGUNDES PASCOAL, AISLAN CRISTINA; SALVADOR, MARCOS JOSE; CINTRA GOMES-MARCONDES, MARIA CRISTINA. Aloe veraand Honey Solution and Their Ethanolic Extraction Solution Could Act on Metastasis-Regulating Processes in Walker 256 Tumor Tissues In Vivo?. NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, v. 73, n. 7, JUN 2020. (10/00714-5, 10/00209-9, 14/13334-7, 17/02739-4, 11/17067-5, 15/03726-8)

WINIEWSKI, VANESSA; SILVA, ADSON S.; ALVAREZ, KATTLEEN D. C.; DE SA, EDUARDO L.; SALVADOR, MARCOS J.; STEFANELLO, MARIA ELIDA A.. ANTIOXIDANT NAPHTHOQUINONES OF Sinningia reitzii FROM SANTA CATARINA STATE, BRAZIL. Química Nova, v. 44, n. 3, p. 284-287, 2021. (15/03726-8)

VERDAN, MARIA HELENA; WINIEWSKI, VANESSA; SCHARF, DILAMARA R.; SALVADOR, MARCOS JOSE; DE SA, EDUARDO L.; STEFANELLO, MARIA ELIDA A.. imeric naphthochromenes and other constituents from Sinningia allagophylla (Gesneriaceae. PHYTOCHEMISTRY LETTERS, v. 42, p. 104-108, APR 2021. (06/06079-4, 12/09101-1, 15/03726-8, 17/03598-5)

MARASCA, SAMUEL; BATTISTI, EDUARDO K.; DURIGON, EMERSON G.; PEIXOTO, NILCE C.; UCZAY, JULIANO; BALDISSEROTTO, BERNARDO; SCHMIDT, DENISE; LORO, VANIA L.; LEITEMPERGER, JOSSIELE W.; SALVADOR, MARCOS J.; et al. Growth, hematology, metabolism, and oxidative parameters of silver catfish (Rhamdia quelen) fed diets containing Lippia alba leaf. Aquaculture, v. 529, DEC 15 2020. (15/03726-8)