Porcine parvovirus VP1/VP2 on a time series epitope mapping: exploring the effects of high hydrostatic pressure on the immune recognition of antigens

Autor(es)

de Souza, Ancelmo Rabelo ^[1] ; Yamin, Marriam ^[1] ; Gava, Danielle ^[2] ; Ciacci Zanella, Janice Reis ^[2] ; Viccari Gatti, Maria Silvia ^[1] ; Sampaio Bonafe, Carlos Francisco ^[1] ; de Lima Neto, Daniel Ferreira ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campimas UNICAMP, Dept Bioquim & Biol Tecidual, Rua Monteiro Lobato, 255, Cidade Univ Zeferino Vaz, BR-13083862 Campinas, SP - Brazil

^[2] Embrapa Suinos & Aves, Lab Virol Suinos, BR-89715899 Concordia, SC - Brazil

^[3] Univ Estadual Campinas UNICAMP, Dept Genet Evolucao & Bioagentes, Inst Biol, Rua Monteiro Lobato, 255, Cidade Univ Zeferino Vaz, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VIROLOGY JOURNAL; v. 16, JUN 3 2019.
• Citações Web of Science: 1

Resumo

Porcine parvovirus (PPV) is a DNA virus that causes reproductive failure in gilts and sows, resulting in embryonic and fetal losses worldwide. Epitope mapping of PPV is important for developing new vaccines. In this study, we used spot synthesis analysis for epitope mapping of the capsid proteins of PPV (NADL-2 strain) and correlated the findings with predictive data from immunoinformatics. The virus was exposed to three conditions prior to inoculation in pigs: native (untreated), high hydrostatic pressure (350MPa for 1h) at room temperature and high hydrostatic pressure (350MPa for 1h) at -18 degrees C, and was compared with a commercial vaccine produced using inactivated PPV. The screening of serum samples detected 44 positive spots corresponding to 20 antigenic sites. Each type of inoculated antigen elicited a distinct epitope set. In silico prediction located linear and discontinuous epitopes in B cells that coincided with several epitopes detected in spot synthesis of sera from pigs that received different preparations of inoculum. The conditions tested elicited antibodies against the VP1/VP2 antigen that differed in relation to the response time and the profile of structurally available regions that were recognized. (AU)

• Processo FAPESP: 08/09835-0 - Potencial vacinal de vírus inativados por alta pressão hidrostática e mapeamento de epítopos de interesse biotecnológico
• Beneficiário: Carlos Francisco Sampaio Bonafe
• Modalidade de apoio: Auxílio à Pesquisa - Regular