Voltammetric determination of 17 beta-estradiol in different matrices using a screen-printed sensor modified with CuPc, Printex 6L carbon and Nafion film

Autor(es)

Wong, Ademar ^{[1, 2]} ; Santos, Anderson Martin ^[1] ; Fava, Elson Luiz ^[1] ; Fatibello-Filho, Orlando ^[1] ; Taboada Sotomayor, Maria Del Pilar ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Chem, BR-13560970 Sao Carlos, SP - Brazil

^[2] State Univ Sao Paulo UNESP, Dept Analyt Chem, Inst Chem, BR-14801970 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Microchemical Journal; v. 147, p. 365-373, JUN 2019.
• Citações Web of Science: 0

Resumo

A screen printed disposable electrode made of carbon ink modified with CuPc, P6LC and Nafion film is proposed as a low cost and simple analytical method for sensitive determination of 17 beta-estradiol in different matrices. The electrochemical behaviour of 17 beta estradiol was evaluated by cyclic voltammetry in the potential range from 0 to 0.55 V at a scan rate of 0.05 V s(-1) using 0.1 mol L-1 phosphate buffer (pH 5.0) as supporting electrolyte. One oxidation peak was observed during anodic potential scanning, at a potential of 0.39 V. Under optimized experimental conditions, using the differential pulse voltammetry (DPV) technique, the proposed sensor showed a linear concentration range for 17 beta-estradiol between 8.0 x 10(-8) to 7.3 x 10(-6) mol L-1, and detection limit of 5.0 x 10(-9) mol L-1. The CuPc-P6LC-Nafion/SPEF sensor showed good selectivity, sensitivity, stability, reproducibility, and repeatability; it is a low-cost disposable device and shows no interference in DPV experiments. The proposed sensor was successful when applied to environmental and synthetic urine samples, with recoveries close to 100%. (AU)

• Processo FAPESP: 14/50945-4 - INCT 2014: Instituto Nacional de Tecnologias Alternativas para Detecção, Avaliação Toxicológica e Remoção de Micropoluentes e Radioativos
• Beneficiário: Maria Valnice Boldrin
• Modalidade de apoio: Auxílio à Pesquisa - Temático