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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

MicroRNA modulated networks of adaptive and innate immune response in pancreatic ductal adenocarcinoma

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Autor(es):
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Felix, Tainara F. [1, 2] ; Lopez Lapa, Rainer M. [2, 3] ; de Carvalho, Marcio [4] ; Bertoni, Natalia [1, 2] ; Tokar, Tomas [5] ; Oliveira, Rogerio A. [6] ; Rodrigues, Maria A. M. [7] ; Hasimoto, Claudia N. [1] ; Oliveira, Walmar K. [1] ; Pelafsky, Leonardo [1] ; Spadella, Cesar T. [1] ; Llanos, Juan C. [1] ; Silva, Giovanni F. [8] ; Lam, Wan L. [9] ; Rogatto, Silvia Regina [10] ; Amorim, Luciana Schultz [11] ; Drigo, Sandra A. [1, 2] ; Carvalho, Robson F. [12] ; Reis, Patricia P. [1, 2]
Número total de Autores: 19
Afiliação do(s) autor(es):
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[1] Sao Paulo State Univ UNESP, Fac Med, Dept Surg & Orthoped, Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Fac Med, Expt Res Unity UNIPEX, Botucatu, SP - Brazil
[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Genet, Botucatu, SP - Brazil
[4] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Vet Clin, Botucatu, SP - Brazil
[5] Univ Hlth Network, Krembil Res Inst, Toronto, ON - Canada
[6] Sao Paulo State Univ UNESP, Inst Biosci, Dept Biostat, Botucatu, SP - Brazil
[7] Sao Paulo State Univ UNESP, Fac Med, Dept Pathol, Botucatu, SP - Brazil
[8] Sao Paulo State Univ UNESP, Fac Med, Dept Clin & Gastroenterol, Botucatu, SP - Brazil
[9] British Columbia Canc Ctr, Genet Unity, Integrat Oncol, Vancouver, BC - Canada
[10] Univ Southern Denmark, Vejle Hosp, Inst Reg Hlth Res, Dept Clin Genet, Odense - Denmark
[11] Inst Pathol Anat, Piracicaba, SP - Brazil
[12] Sao Paulo State Univ UNESP, Inst Biosci, Dept Morphol, Botucatu, SP - Brazil
Número total de Afiliações: 12
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 14, n. 5 MAY 31 2019.
Citações Web of Science: 2
Resumo

Despite progress in treatment strategies, only similar to 24% of pancreatic ductal adenocarcinoma (PDAC) patients survive > 1 year. Our goal was to elucidate deregulated pathways modulated by microRNAs (miRNAs) in PDAC and Vater ampulla (AMP) cancers. Global miRNA expression was identified in 19 PDAC, 6 AMP and 25 paired, histologically normal pancreatic tissues using the GeneChip 4.0 miRNA arrays. Computational approaches were used for miRNA target prediction/identification of miRNA-regulated pathways. Target gene expression was validated in 178 pancreatic cancer and 4 pancreatic normal tissues from The Cancer Genome Atlas (TCGA). 20 miRNAs were significantly deregulated (FC >= 2 and p< 0.05) (15 down-and 5 up-regulated) in PDAC. miR-216 family (miR-216a-3p, miR-216a5p, miR-216b-3p and miR-216b-5p) was consistently down-regulated in PDAC. miRNA-modulated pathways are associated with innate and adaptive immune system responses in PDAC. AMP cancers showed 8 down-and 1 up-regulated miRNAs (FDR p< 0.05). Most enriched pathways (p< 0.01) were RAS and Nerve Growth Factor signaling. PDAC and AMP display different global miRNA expression profiles and miRNA regulated networks/tumorigenesis pathways. The immune response was enriched in PDAC, suggesting the existence of immune checkpoint pathways more relevant to PDAC than AMP. (AU)

Processo FAPESP: 14/00367-4 - MicroRNoma do carcinoma de pâncreas
Beneficiário:Tainara Francini Felix
Modalidade de apoio: Bolsas no Brasil - Mestrado
Processo FAPESP: 16/03905-2 - MicroRNAs circulantes em cânceres do fígado, pâncreas e vias biliares: identificação de biomarcadores potenciais e caracterização funcional
Beneficiário:Patricia Pintor dos Reis
Modalidade de apoio: Auxílio à Pesquisa - Regular