| Full text | |
| Author(s): Show less - |
Felix, Tainara F.
[1, 2]
;
Lopez Lapa, Rainer M.
[2, 3]
;
de Carvalho, Marcio
[4]
;
Bertoni, Natalia
[1, 2]
;
Tokar, Tomas
[5]
;
Oliveira, Rogerio A.
[6]
;
Rodrigues, Maria A. M.
[7]
;
Hasimoto, Claudia N.
[1]
;
Oliveira, Walmar K.
[1]
;
Pelafsky, Leonardo
[1]
;
Spadella, Cesar T.
[1]
;
Llanos, Juan C.
[1]
;
Silva, Giovanni F.
[8]
;
Lam, Wan L.
[9]
;
Rogatto, Silvia Regina
[10]
;
Amorim, Luciana Schultz
[11]
;
Drigo, Sandra A.
[1, 2]
;
Carvalho, Robson F.
[12]
;
Reis, Patricia P.
[1, 2]
Total Authors: 19
|
| Affiliation: Show less - | [1] Sao Paulo State Univ UNESP, Fac Med, Dept Surg & Orthoped, Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Fac Med, Expt Res Unity UNIPEX, Botucatu, SP - Brazil
[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Genet, Botucatu, SP - Brazil
[4] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Vet Clin, Botucatu, SP - Brazil
[5] Univ Hlth Network, Krembil Res Inst, Toronto, ON - Canada
[6] Sao Paulo State Univ UNESP, Inst Biosci, Dept Biostat, Botucatu, SP - Brazil
[7] Sao Paulo State Univ UNESP, Fac Med, Dept Pathol, Botucatu, SP - Brazil
[8] Sao Paulo State Univ UNESP, Fac Med, Dept Clin & Gastroenterol, Botucatu, SP - Brazil
[9] British Columbia Canc Ctr, Genet Unity, Integrat Oncol, Vancouver, BC - Canada
[10] Univ Southern Denmark, Vejle Hosp, Inst Reg Hlth Res, Dept Clin Genet, Odense - Denmark
[11] Inst Pathol Anat, Piracicaba, SP - Brazil
[12] Sao Paulo State Univ UNESP, Inst Biosci, Dept Morphol, Botucatu, SP - Brazil
Total Affiliations: 12
|
| Document type: | Journal article |
| Source: | PLoS One; v. 14, n. 5 MAY 31 2019. |
| Web of Science Citations: | 2 |
| Abstract | |
Despite progress in treatment strategies, only similar to 24% of pancreatic ductal adenocarcinoma (PDAC) patients survive > 1 year. Our goal was to elucidate deregulated pathways modulated by microRNAs (miRNAs) in PDAC and Vater ampulla (AMP) cancers. Global miRNA expression was identified in 19 PDAC, 6 AMP and 25 paired, histologically normal pancreatic tissues using the GeneChip 4.0 miRNA arrays. Computational approaches were used for miRNA target prediction/identification of miRNA-regulated pathways. Target gene expression was validated in 178 pancreatic cancer and 4 pancreatic normal tissues from The Cancer Genome Atlas (TCGA). 20 miRNAs were significantly deregulated (FC >= 2 and p< 0.05) (15 down-and 5 up-regulated) in PDAC. miR-216 family (miR-216a-3p, miR-216a5p, miR-216b-3p and miR-216b-5p) was consistently down-regulated in PDAC. miRNA-modulated pathways are associated with innate and adaptive immune system responses in PDAC. AMP cancers showed 8 down-and 1 up-regulated miRNAs (FDR p< 0.05). Most enriched pathways (p< 0.01) were RAS and Nerve Growth Factor signaling. PDAC and AMP display different global miRNA expression profiles and miRNA regulated networks/tumorigenesis pathways. The immune response was enriched in PDAC, suggesting the existence of immune checkpoint pathways more relevant to PDAC than AMP. (AU) | |
| FAPESP's process: | 16/03905-2 - Circulating microRNAs in cancers of the liver, pancreas and the biliary tree: identification of potential biomarkers and functional characterization |
| Grantee: | Patricia Pintor dos Reis |
| Support type: | Regular Research Grants |
| FAPESP's process: | 14/00367-4 - MicroRNome of pancreatic câncer |
| Grantee: | Tainara Francini Felix |
| Support type: | Scholarships in Brazil - Master |