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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

MicroRNA modulated networks of adaptive and innate immune response in pancreatic ductal adenocarcinoma

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Author(s):
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Felix, Tainara F. [1, 2] ; Lopez Lapa, Rainer M. [2, 3] ; de Carvalho, Marcio [4] ; Bertoni, Natalia [1, 2] ; Tokar, Tomas [5] ; Oliveira, Rogerio A. [6] ; Rodrigues, Maria A. M. [7] ; Hasimoto, Claudia N. [1] ; Oliveira, Walmar K. [1] ; Pelafsky, Leonardo [1] ; Spadella, Cesar T. [1] ; Llanos, Juan C. [1] ; Silva, Giovanni F. [8] ; Lam, Wan L. [9] ; Rogatto, Silvia Regina [10] ; Amorim, Luciana Schultz [11] ; Drigo, Sandra A. [1, 2] ; Carvalho, Robson F. [12] ; Reis, Patricia P. [1, 2]
Total Authors: 19
Affiliation:
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[1] Sao Paulo State Univ UNESP, Fac Med, Dept Surg & Orthoped, Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Fac Med, Expt Res Unity UNIPEX, Botucatu, SP - Brazil
[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Genet, Botucatu, SP - Brazil
[4] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Vet Clin, Botucatu, SP - Brazil
[5] Univ Hlth Network, Krembil Res Inst, Toronto, ON - Canada
[6] Sao Paulo State Univ UNESP, Inst Biosci, Dept Biostat, Botucatu, SP - Brazil
[7] Sao Paulo State Univ UNESP, Fac Med, Dept Pathol, Botucatu, SP - Brazil
[8] Sao Paulo State Univ UNESP, Fac Med, Dept Clin & Gastroenterol, Botucatu, SP - Brazil
[9] British Columbia Canc Ctr, Genet Unity, Integrat Oncol, Vancouver, BC - Canada
[10] Univ Southern Denmark, Vejle Hosp, Inst Reg Hlth Res, Dept Clin Genet, Odense - Denmark
[11] Inst Pathol Anat, Piracicaba, SP - Brazil
[12] Sao Paulo State Univ UNESP, Inst Biosci, Dept Morphol, Botucatu, SP - Brazil
Total Affiliations: 12
Document type: Journal article
Source: PLoS One; v. 14, n. 5 MAY 31 2019.
Web of Science Citations: 2
Abstract

Despite progress in treatment strategies, only similar to 24% of pancreatic ductal adenocarcinoma (PDAC) patients survive > 1 year. Our goal was to elucidate deregulated pathways modulated by microRNAs (miRNAs) in PDAC and Vater ampulla (AMP) cancers. Global miRNA expression was identified in 19 PDAC, 6 AMP and 25 paired, histologically normal pancreatic tissues using the GeneChip 4.0 miRNA arrays. Computational approaches were used for miRNA target prediction/identification of miRNA-regulated pathways. Target gene expression was validated in 178 pancreatic cancer and 4 pancreatic normal tissues from The Cancer Genome Atlas (TCGA). 20 miRNAs were significantly deregulated (FC >= 2 and p< 0.05) (15 down-and 5 up-regulated) in PDAC. miR-216 family (miR-216a-3p, miR-216a5p, miR-216b-3p and miR-216b-5p) was consistently down-regulated in PDAC. miRNA-modulated pathways are associated with innate and adaptive immune system responses in PDAC. AMP cancers showed 8 down-and 1 up-regulated miRNAs (FDR p< 0.05). Most enriched pathways (p< 0.01) were RAS and Nerve Growth Factor signaling. PDAC and AMP display different global miRNA expression profiles and miRNA regulated networks/tumorigenesis pathways. The immune response was enriched in PDAC, suggesting the existence of immune checkpoint pathways more relevant to PDAC than AMP. (AU)

FAPESP's process: 16/03905-2 - Circulating microRNAs in cancers of the liver, pancreas and the biliary tree: identification of potential biomarkers and functional characterization
Grantee:Patricia Pintor dos Reis
Support type: Regular Research Grants
FAPESP's process: 14/00367-4 - MicroRNome of pancreatic câncer
Grantee:Tainara Francini Felix
Support type: Scholarships in Brazil - Master