Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone

Autor(es)

Fallah, Hamideh P. ^[1] ; Tovo-Neto, Aldo ^{[1, 2, 3]} ; Yeung, Edward C. ^[1] ; Nobrega, Rafael H. ^[2] ; Habibi, Hamid R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Calgary, Dept Biol Sci, 2500 Univ Dr NW, Calgary, AB T2N 1N4 - Canada

^[2] Sao Paulo State Univ, Dept Morphol, Reprod & Mol Biol Grp, Botucatu, SP - Brazil

^[3] Sao Paulo State Univ Unesp, Aquaculture Program, Jaboticabal, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular and Cellular Endocrinology; v. 492, JUL 15 2019.
• Citações Web of Science: 1

Resumo

Control of testicular development is multifactorial and involves a number of hypothalamic, hypophyseal and peripheral hormones. Here, we investigated direct action of zebrafish gonadotropin-inhibitory hormone (zGnih) which is expressed in the testis, on spermatogenesis in zebrafish, in vitro. Treatment with zGnih at the lower doses (10 and 100 nM) inhibited gonadotropin-induced spermatids/spermatozoa (SPD/SPZ) production. However, at the highest dose (1000 nM), zGnih increased basal number of SPD/SPZ and showed paradoxical effect. The effects of zGnih on testosterone and SPD/SPZ production was blocked in the presence of androgen receptor antagonist, flutamide (FLU). A number of transcripts were also measured to better understand zGnih mechanisms of action on zebrafish spermatogenesis. Our results provide strong support for the hypothesis that locally produced zGnih is a component of the complex multifactorial system that regulates testicular development and function in adult zebrafish, in part, by changes in testicular steroidogenesis and regulation of gonadotropin-induced response. (AU)

• Processo FAPESP: 14/07620-7 - Banco de espermatogônias tronco em peixes para conservação do genótipo de espécies ameaçadas e de interesse econômico
• Beneficiário: Rafael Henrique Nóbrega
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 17/09218-0 - Efeitos da insuficiência do hormônio tireoidiano induzida pelo metilmazol em zebrafish (Danio rerio)
• Beneficiário: Aldo Tovo Neto
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 14/16280-5 - Controle endócrino e molecular envolvidos na diferenciação gonadal de zebrafish (Danio rerio)
• Beneficiário: Aldo Tovo Neto
• Modalidade de apoio: Bolsas no Brasil - Doutorado