Conjugated Polymers Blended with Lipids and Galactosidase as Langmuir-Blodgett Films To Control the Biosensing Properties of Nanostructured Surfaces

Autor(es)

Araujo, Felipe Tejada ^[1] ; Peres, Laura Oliveira ^[1] ; Caseli, Luciano ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, BR-09913030 Diadema, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Langmuir; v. 35, n. 22, p. 7294-7303, JUN 4 2019.
• Citações Web of Science: 3

Resumo

The structure of enzymes must be conserved when incorporated in nanoelectronic devices because their activity determines the function of the device as sensors. Among the systems that can retain their conformational structures, Langmuir-Blodgett (LB) films can be useful to exploit the construction of bioelectronic devices organized at the molecular level because biological and polymeric materials can be coupled as ultrathin films for biosensors and actuators. In this paper, we immobilized a beta-galactosidase enzyme in the LB films of stearic acid and the conjugated polymer poly{[}(9,9-dioctylfluorene)-co-thiophene]. After the characterization of the floating films using tensiometry, vibrational spectroscopy, and Brewster angle microscopy, they were transferred to solid supports as LB films, and the catalytic activity of the enzyme could be preserved as analyzed using UV-vis spectroscopy. We noted that the presence of a supramolecular structure formed in the LB films not only conserved the enzyme activity but also exhibited regular and distinctive output signals in all molecular architectures employed in this work. These results are related to the synergism between the compounds on the active layer associated with a surface morphology that facilitated the analyte diffusion because of an adequate molecular accommodation of all components. This work then demonstrates the viability of employing LB films composed of lipids, enzymes, and synthetic polymers as devices for biosensing applications. (AU)

• Processo FAPESP: 19/03239-0 - Interfaces nanoestruturadas para a investigação de substâncias bioativas em modelos de membrana celular e para a construção de dispositivos optoeletrônicos enzimáticos
• Beneficiário: Luciano Caseli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/50869-6 - INCT 2014: em Eletrônica Orgânica INEO
• Beneficiário: Roberto Mendonça Faria
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/23446-0 - Superfícies inteligentes nanoestruturadas e bioinspiradas: investigação de interações em modelos de membrana e produção de dispositivos para biossensores e análise ambiental.
• Beneficiário: Luciano Caseli
• Modalidade de apoio: Auxílio à Pesquisa - Regular