Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Negative impact of Novaluron on the nontarget insect Bombyx mori (Lepidoptera: Bombycidae)

Texto completo
Autor(es):
Santorum, Marilucia [1] ; Costa Brancalhao, Rose Meire [2] ; Bittencourt Guimaraes, Ana Tereza [2] ; Padovani, Carlos Roberto [3] ; Tettamanti, Gianluca [4] ; dos Santos, Daniela Carvalho [1, 5]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Morphol, Lab Insects, Botucatu, SP - Brazil
[2] State Univ Western Parana, UNIOESTE, Ctr Biol Sci & Hlth, Cascavel, PR - Brazil
[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Biostat, Botucatu, SP - Brazil
[4] Univ Insubria, Dept Biotechnol & Life Sci, Varese - Italy
[5] Univ Estadual Paulista, Electron Microscopy Ctr, Inst Biosci Botucatu, Sao Paulo, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Environmental Pollution; v. 249, p. 82-90, JUN 2019.
Citações Web of Science: 1
Resumo

Due to increased use of agrochemicals and growing concerns about ecotoxicology, the development of new insecticides, moving away from those with neurotoxic and broad spectrum effects towards insecticides that are safer for the environment and nontarget beneficial species, has been a research priority. Novaluron stands out among these newer insecticides, is an insect growth regulator that is used for the control of insect pests in crops grown close to mulberry plantations. Mulberry serves as food for the silkworm Bombyx mori, which is a nontarget insect of great economic importance to silk production. We investigated the lethal and sublethal effects of Novaluron on the development of B. mori. Larvae were segregated into experimental groups: the control groups (CGs) and the treatment groups (TGs), which were treated with the Novaluron concentration of 0.15 mL/L. Following exposure, we analyzed: larval mortality, changes in the insect life cicle and cytotoxic effects on the midgut cells. This is the first report about the Novaluron's effects on B.mori. We detected rupture in the integument, complete cessation of feeding, late development, incomplete ecdysis and production of defective cocoons. After 240 h of exposure, there was 100% mortality in TG larvae exposed in the 3rd instar and 20% mortality from larvae exposed in the 5th instar. Cytotoxic effects was observed, such as dilation of cells, emission of cytoplasmic protrusions, extreme rarefaction of the cytoplasm and nuclei, dilation of the endoplasmic reticulum in addition to changes in mitochondria, the presence of large digestive vacuoles and intercellular spaces and the presence of active caspase. Novaluron exposure impairs the midgut and may affect the physiological functions of this organ. Novaluron additionally compromises several phases of insect development, indicating the importance of toxicology studies that utilize different life stages of nontarget species to evaluate the safe use of insecticides. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 15/07119-9 - Avaliação da toxicidade do inseticida Novalurom em Bombyx mori (Lepidoptera: Bombycidae)
Beneficiário:Marilucia Santorum
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 17/16714-3 - ANÁLISE DA APOPTOSE NO INTESTINO MÉDIO DE Bombyx mori (LEPIDOPTERA: BOMBYCIDAE) TRATADO COM NOVALUROM
Beneficiário:Marilucia Santorum
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado