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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Negative impact of Novaluron on the nontarget insect Bombyx mori (Lepidoptera: Bombycidae)

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Author(s):
Santorum, Marilucia [1] ; Costa Brancalhao, Rose Meire [2] ; Bittencourt Guimaraes, Ana Tereza [2] ; Padovani, Carlos Roberto [3] ; Tettamanti, Gianluca [4] ; dos Santos, Daniela Carvalho [1, 5]
Total Authors: 6
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Morphol, Lab Insects, Botucatu, SP - Brazil
[2] State Univ Western Parana, UNIOESTE, Ctr Biol Sci & Hlth, Cascavel, PR - Brazil
[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Biostat, Botucatu, SP - Brazil
[4] Univ Insubria, Dept Biotechnol & Life Sci, Varese - Italy
[5] Univ Estadual Paulista, Electron Microscopy Ctr, Inst Biosci Botucatu, Sao Paulo, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Environmental Pollution; v. 249, p. 82-90, JUN 2019.
Web of Science Citations: 1
Abstract

Due to increased use of agrochemicals and growing concerns about ecotoxicology, the development of new insecticides, moving away from those with neurotoxic and broad spectrum effects towards insecticides that are safer for the environment and nontarget beneficial species, has been a research priority. Novaluron stands out among these newer insecticides, is an insect growth regulator that is used for the control of insect pests in crops grown close to mulberry plantations. Mulberry serves as food for the silkworm Bombyx mori, which is a nontarget insect of great economic importance to silk production. We investigated the lethal and sublethal effects of Novaluron on the development of B. mori. Larvae were segregated into experimental groups: the control groups (CGs) and the treatment groups (TGs), which were treated with the Novaluron concentration of 0.15 mL/L. Following exposure, we analyzed: larval mortality, changes in the insect life cicle and cytotoxic effects on the midgut cells. This is the first report about the Novaluron's effects on B.mori. We detected rupture in the integument, complete cessation of feeding, late development, incomplete ecdysis and production of defective cocoons. After 240 h of exposure, there was 100% mortality in TG larvae exposed in the 3rd instar and 20% mortality from larvae exposed in the 5th instar. Cytotoxic effects was observed, such as dilation of cells, emission of cytoplasmic protrusions, extreme rarefaction of the cytoplasm and nuclei, dilation of the endoplasmic reticulum in addition to changes in mitochondria, the presence of large digestive vacuoles and intercellular spaces and the presence of active caspase. Novaluron exposure impairs the midgut and may affect the physiological functions of this organ. Novaluron additionally compromises several phases of insect development, indicating the importance of toxicology studies that utilize different life stages of nontarget species to evaluate the safe use of insecticides. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 17/16714-3 - ANALYSIS OF APOPTOSIS IN THE MIDGUT OF Bombyx mori (LEPIDOPTERA: BOMBYCIDAE) TREATED WITH NOVALUROM
Grantee:Marilucia Santorum
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 15/07119-9 - Toxicity evaluation of the insecticide Novaluron in Bombyx mori (Lepidoptera: Bombycidae)
Grantee:Marilucia Santorum
Support type: Scholarships in Brazil - Doctorate