Oral administration of EPA-rich oil impairs collagen reorganization due to elevated production of IL-10 during skin wound healing in mice

Autor(es):Mostrar menos -

Burger, Beatriz ^[1] ; Kuhl, Carolina M. C. ^[1] ; Candreva, Thamiris ^[1] ; Cardoso, Renato da S. ^[1] ; Silva, Jessica R. ^{[1, 2]} ; Castelucci, Bianca G. ^[3] ; Consonni, Silvio R. ^[3] ; Fisk, Helena L. ^[4] ; Calder, Philip C. ^{[5, 6, 4]} ; Vinolo, Marco Aurelio R. ^[2] ; Rodrigues, Hosana G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Appl Sci, Lab Nutrients & Tissue Repair, Limeira, SP - Brazil

^[2] Univ Estadual Campinas, Dept Genet Evolut Microbiol & Immunol, Inst Biol, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil

^[4] Univ Southampton, Fac Med Human Dev & Hlth, Southampton, Hants - England

^[5] Univ Southampton, Southampton, Hants - England

^[6] Univ Hosp Southampton NHS Fdn Trust, NIHR Southampton Biomed Res Ctr, Southampton, Hants - England

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, JUN 24 2019.
• Citações Web of Science: 1

Resumo

Wound healing is an essential process for organism survival. Some fatty acids have been described as modulators of wound healing. However, the role of omega-3 fatty acids is unclear. In the present work, we investigate the effects of oral administration of eicosapentaenoic acid (EPA)-rich oil on wound healing in mice. After 4 weeks of EPA-rich oil supplementation (2 g/kg of body weight), mice had increased serum concentrations of EPA (20: 5 omega-3) (6-fold) and docosahexaenoic acid (DHA; 22: 6 omega-3) (33%) in relation to control mice. Omega-3 fatty acids were also incorporated into skin in the EPA fed mice. The wound healing process was delayed at the 3rd and 7th days after wounding in mice that received EPA-rich oil when compared to control mice but there was no effect on the total time required for wound closure. Collagen reorganization, that impacts the quality of the wound tissue, was impaired after EPA-rich oil supplementation. These effects were associated with an increase of M2 macrophages (twice in relation to control animals) and interleukin-10 (IL-10) concentrations in tissue in the initial stages of wound healing. In the absence of IL-10 (IL-10(-/-) mice), wound closure and organization of collagen were normalized even when EPA was fed, supporting that the deleterious effects of EPA-rich oil supplementation were due to the excessive production of IL-10. In conclusion, oral administration of EPA-rich oil impairs the quality of wound healing without affecting the wound closure time likely due to an elevation of the anti-inflammatory cytokine IL-10. (AU)

• Processo FAPESP: 13/06810-4 - Mecanismos de ação dos ácidos graxos ômega-3 e ômega-6 no processo de reparo tecidual: enfoque neuro-imunológico
• Beneficiário: Hosana Gomes Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 18/11037-6 - O envolvimento dos macrófagos nos efeitos da suplementação com ácidos graxos ômega-3 sobre a cicatrização de feridas: da bancada para o leito do paciente
• Beneficiário: Hosana Gomes Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/15127-9 - EMU concedido no processo: 2013/06810-4 - citômetro de fluxo BD Accury
• Beneficiário: Hosana Gomes Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários