Effects of resveratrol on the differentiation fate of neural progenitor cells of mouse embryos infected with Trypanosoma cruzi

Autor(es):Mostrar menos -

Fracasso, Mateus ^[1] ; Bottari, Nathieli B. ^[1] ; da Silva, Anielen D. ^[1] ; Grando, Thirssa H. ^{[2, 3]} ; Pillat, Micheli M. ^[4] ; Ulrich, Henning ^[4] ; Vidal, Tais ^[1] ; de Andrade, Cinthia M. ^{[1, 2]} ; Monteiro, Silvia G. ^[2] ; Nascimento, Luiz Flavio N. ^[5] ; Miletti, Luiz Claudio ^[5] ; da Silva, Aleksandro Schafer ^[6]

Afiliação do(s) autor(es)

^[1] Univ Fed Santa Maria, Dept Biochem & Mol Biol, Grad Program Toxicol Biochem, Santa Maria, RS - Brazil

^[2] Univ Fed Santa Maria, Dept Parasitol Microbiol & Immunol, Grad Program Vet Med, Santa Maria, RS - Brazil

^[3] Inst Fed Farroupilha IFFar, Campus Frederico, Westphalen, RS - Brazil

^[4] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo, SP - Brazil

^[5] Univ Estado Santa Catarina UDESC, Grad Program Anim Sci, Lages, SC - Brazil

^[6] Univ Estado Santa Catarina UDESC, Dept Anim Sci, Chapeco, SC - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Microbial Pathogenesis; v. 132, p. 156-161, JUL 2019.
• Citações Web of Science: 1

Resumo

Chagas disease (CD) affecting about 7 million people is caused by the flagellate protozoan Trypanosoma cruzi. The central nervous system (CNS) is an important site for T. cruzi persistence in the host during the chronic phase of infection, because the protozoan may pass the blood-brain barrier and may cause motor and cognitive neuronal damage. Thinking about avoiding or minimizing these negative effects, it is hypothesized that resveratrol (RSV), a component with several medicinal properties has beneficial effects on the CNS. The objective of this study was to investigate, whether T. cruzi infection interferes with neurogenesis and gliogenesis of embryos of infected mice females, and whether RSV would be able to avoid or minimize these changes caused by CD. RSV is a polyphenol found in grapes and widely studied for its neuroprotective and antioxidant properties. In addition, we investigated the role caused by the parasite during congenital infection and CNS development. Embryos and their brains were PCR-positive for T. cruzi. For this study, NPCs obtained from telencephalon of infected and uninfected embryos and were cultured in presence of resveratrol for forming neurospheres. The results demonstrated that the congenital transmission of T. cruzi influences CNS formation and neural fate, decreasing the number of neuroespheres and causing an elongation in the phases of the cell cycle. In addition, the parasite promoted an increase in neugliogenesis. Resveratrol was neuroprotective and prevented negative effects of the infection. Thus, we suggest the use of resveratrol as a therapeutic target for the treatment of neuroinflammation or as neuroprotective agent during Chagas disease, as it improves gliogenesis and restores neural migration. (AU)

• Processo FAPESP: 12/50880-4 - Células-tronco: dos papéis de receptores de cininas e purinas às aplicações terapêuticas
• Beneficiário: Alexander Henning Ulrich
• Modalidade de apoio: Auxílio à Pesquisa - Temático