Selective antibacterial activity of the cationic peptide PaDBS1R6 against Gram-negative bacteria

Autor(es):Mostrar menos -

Fensterseifer, Isabel C. M. ^{[1, 2]} ; Felicio, Mario R. ^[3] ; Alves, Eliane S. F. ^[4] ; Cardoso, Marlon H. ^{[1, 2, 5]} ; Torres, Marcelo D. T. ^{[6, 7, 8]} ; Matos, Carolina O. ^[4] ; Silva, Osmar N. ^[5] ; Lu, Timothy K. ^{[9, 10]} ; Freire, V, Mauricio ; Neves, Natan C. ^[11] ; Goncalves, Sonia ^[3] ; Liao, Luciano M. ^[4] ; Santos, Nuno C. ^[3] ; Porto, William F. ^{[5, 12]} ; de la Fuente-Nunez, Cesar ^{[6, 7, 8]} ; Franco, Octavio L. ^{[2, 5, 11]}

Afiliação do(s) autor(es):Mostrar menos -

^[1] Univ Catolica Brasilia, Posgrad Ciencias Genom & Biotecnol, Ctr Anal Proteom & Bioquim, Brasilia, DF - Brazil

^[2] Univ Catolica Brasilia, Programa Posgrad Patol Mol, Brasilia, DF - Brazil

^[3] Univ Lisbon, Fac Med, Inst Med Mol, Lisbon - Portugal

^[4] Univ Fed Goias, Inst Quim, Lab RMN, Goiania, Go - Brazil

^[5] Univ Catolica Dom Bosco, Posgrad Biotecnol, S Inova Biotech, Campo Grande, MS - Brazil

^[6] Univ Penn, Dept Microbiol, Perelman Sch Med, Philadelphia, PA 19104 - USA

^[7] Univ Penn, Dept Psychiat, Perelman Sch Med, Philadelphia, PA 19104 - USA

^[8] Univ Penn, Dept Bioengn, Philadelphia, PA 19104 - USA

^[9] Broad Inst MIT & Harvard, Cambridge, MA 02142 - USA

^[10] MIT, Dept Elect Engn & Comp Sci, Dept Biol Engn, Synthet Biol Grp, Synthet Biol Ctr, Ctr Microbiome Informat & Therapeut, Res Lab Elect, Cambridge, MA 02139 - USA

^[11] Freire, Mauricio, V, Univ Catolica Brasilia, Posgrad Ciencias Genom & Biotecnol, Ctr Anal Proteom & Bioquim, Brasilia, DF - Brazil

^[12] Porto Reports, Brasilia, DF - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES; v. 1861, n. 7, p. 1375-1387, JUL 1 2019.
• Citações Web of Science: 5

Resumo

Infections caused by Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa foremost among them, constitute a major worldwide health problem. Bioinformatics methodologies are being used to rationally design new antimicrobial peptides, a potential alternative for treating these infections. One of the algorithms used to develop antimicrobial peptides is the Joker, which was used to design the peptide PaDBS1R6. This study evaluates the antibacterial activities of PaDBS1R6 in vitro and in vivo, characterizes the peptide interaction to target membranes, and investigates the PaDBS1R6 structure in contact with mimetic vesicles. Moreover, we demonstrate that PaDBS1R6 exhibits selective antimicrobial activity against Gram-negative bacteria. In the presence of negatively charged and zwitterionic lipids the structural arrangement of PaDBS1R6 transits from random coil to alpha-helix, as characterized by circular dichroism. The tertiary structure of PaDBS1R6 was determined by NMR in zwitterionic dodecylphosphocholine (DPC) micelles. In conclusion, PaDBS1R6 is a candidate for the treatment of nosocomial infections caused by Gram-negative bacteria, as template for producing other antimicrobial agents. (AU)

• Processo FAPESP: 16/24413-0 - Peptídeos anfipáticos catiônicos antimicrobianos e antibiofilmes
• Beneficiário: Marcelo Der Torossian Torres
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado