| Texto completo | |
| Autor(es): |
Martins Nascimento, Paloma Andrade
[1]
;
Brandao Pereira, Jorge Fernando
[1]
;
Santos-Ebinuma, Valeria de Carvalho
[1]
Número total de Autores: 3
|
| Afiliação do(s) autor(es): | [1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Bioproc & Biotechnol, Rodovia Araraquara Jau Km 01, BR-14800903 Araraquara, SP - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Bioprocess and Biosystems Engineering; v. 42, n. 7, p. 1235-1246, JUL 2019. |
| Citações Web of Science: | 1 |
| Resumo | |
This work studied the effect of the cation alkyl chain length of 1-alkyl-n-methylimidazolium chloride ({[}C(n)mim]Cl)-based ILs on the activity of Aspergillus niger lipase. First, the lipase activity in the presence of different ILs concentration over time was determined. ILs with shorter cation alkyl side chain length, namely {[}C(4)mim]Cl and {[}C(6)mim]Cl, promoted an increase of lipase activity; while, {[}C(8)mim]Cl, depending on its concentration, maintained or decreased the enzyme activity. In the presence of ILs with longer cation alkyl chain length, i.e., {[}C(10)mim]Cl and {[}C(12)mim]Cl, the lipase relative activity was reduced with 0.1 (%v/v) and until suppressed ({[}C(12)mim]Cl at 0.3 (%v/v)) as a result of irreversible changes in its secondary structure. Fluorescence and circular dichroism spectroscopy analysis confirmed the results achieved. These findings show that {[}C(n)mim]Cl-based ILs can exert different behavior on the lipase' activity (enhance, maintain or even inhibit) and structural conformation, depending on the cation alkyl chain length and their relative concentration. (AU) | |
| Processo FAPESP: | 14/16424-7 - Otimização e aumento de escala do processo de extração líquido-líquido com líquidos iônicos (LIs) como ferramenta de separação sustentável do biofármaco antileucêmico L-asparaginase (ASPase) |
| Beneficiário: | Jorge Pereira |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |