| Full text | |
| Author(s): |
Martins Nascimento, Paloma Andrade
[1]
;
Brandao Pereira, Jorge Fernando
[1]
;
Santos-Ebinuma, Valeria de Carvalho
[1]
Total Authors: 3
|
| Affiliation: | [1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Bioproc & Biotechnol, Rodovia Araraquara Jau Km 01, BR-14800903 Araraquara, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Bioprocess and Biosystems Engineering; v. 42, n. 7, p. 1235-1246, JUL 2019. |
| Web of Science Citations: | 1 |
| Abstract | |
This work studied the effect of the cation alkyl chain length of 1-alkyl-n-methylimidazolium chloride ({[}C(n)mim]Cl)-based ILs on the activity of Aspergillus niger lipase. First, the lipase activity in the presence of different ILs concentration over time was determined. ILs with shorter cation alkyl side chain length, namely {[}C(4)mim]Cl and {[}C(6)mim]Cl, promoted an increase of lipase activity; while, {[}C(8)mim]Cl, depending on its concentration, maintained or decreased the enzyme activity. In the presence of ILs with longer cation alkyl chain length, i.e., {[}C(10)mim]Cl and {[}C(12)mim]Cl, the lipase relative activity was reduced with 0.1 (%v/v) and until suppressed ({[}C(12)mim]Cl at 0.3 (%v/v)) as a result of irreversible changes in its secondary structure. Fluorescence and circular dichroism spectroscopy analysis confirmed the results achieved. These findings show that {[}C(n)mim]Cl-based ILs can exert different behavior on the lipase' activity (enhance, maintain or even inhibit) and structural conformation, depending on the cation alkyl chain length and their relative concentration. (AU) | |
| FAPESP's process: | 14/16424-7 - Optimization and scale-up of liquid-liquid extraction process with ionic liquids (ILs) as a sustainable tool for the separation of the anti-leukemia biopharmaceutical L-asparaginase (ASPase) |
| Grantee: | Jorge Pereira |
| Support Opportunities: | Research Grants - Young Investigators Grants |