Schistosoma mansoni venom allergen-like proteins: phylogenetic relationships, stage-specific transcription and tissue localization as predictors of immunological cross-reactivity

Autor(es):Mostrar menos -

Farias, Leonardo P. ^{[1, 2]} ; Chalmers, Lain W. ^[3] ; Perally, Samirah ^[3] ; Rofatto, Henrique K. ^[1] ; Jackson, Colin J. ^[3] ; Brown, Martha ^[3] ; Khouri, I, Mariana ; Barbosa, Mayra M. F. ^{[1, 4]} ; Hensbergen, Paul J. ^[5] ; Hokke, Cornelis H. ^[6] ; Leite, Luciana C. C. ^[1] ; Hoffmann, Karl F. ^[3]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Ctr Biotecnol, Av Vital Brasil 1500, BR-05503900 Sao Paulo, SP - Brazil

^[2] I, Fundacao Oswaldo Cruz FIOCRUZ, Inst Goncalo Moniz, Rua Waldemar Falcao, Salvador, BA - Brazil

^[3] Aberystwyth Univ, Inst Biol Environm & Rural Sci, Aberystwyth SY23 3FG, Dyfed - Wales

^[4] Univ Sao Paulo, Programa Posgrad Interunidades Biotecnol, Sao Paulo, SP - Brazil

^[5] Leiden Univ, Ctr Prote & Metabol, Med Ctr, POB 9600, NL-2300 RC Leiden - Netherlands

^[6] Leiden Univ, Dept Parasitol, Med Ctr, POB 9600, NL-2300 RC Leiden - Netherlands

• Tipo de documento: Artigo Científico
• Fonte: International Journal for Parasitology; v. 49, n. 8, p. 593-599, JUL 2019.
• Citações Web of Science: 0

Resumo

Schistosoma mansoni venom allergen-like proteins (SmVALs) are part of a diverse protein superfamily partitioned into two groups (group 1 and group 2). Phylogenetic analyses of group 1 SmVALs revealed that members could be segregated into subclades (A-D); these subclades share similar gene expression patterns across the parasite lifecycle and immunological cross-reactivity. Furthermore, whole-mount in situ hybridization demonstrated that the phylogenetically, transcriptionally and immunologically-related SmVAL4, 10, 18 and 19 (subclade C) were all localized to the pre-acetabular glands of immature cercariae. Our results suggest that SmVAL group 1 phylogenetic relationships, stage-specific transcriptional profiles and tissue localization are predictive of immunological cross-reactivity. (C) 2019 The Authors. Published by Elsevier Ltd on behalf of Australian Society for parasitology. (AU)

• Processo FAPESP: 12/23124-4 - Investigação dos mecanismos efetores da vacina atenuada de Schistosoma mansoni utilizando Systems Vaccinology
• Beneficiário: Luciana Cezar de Cerqueira Leite
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/57946-5 - Investigating the immunomodulatory properties of venom allergen like proteins from Schistosoma mansoni
• Beneficiário: Leonardo Paiva Farias
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 07/07685-8 - Caracterização molecular e bioquímica das Nucleotídeo Pirofosfatases/Fosfodiesterases (NPPs) de Schistosoma mansoni e investigação como antígenos vacinais
• Beneficiário: Henrique Krambeck Rofatto
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 00/11624-5 - Embriologia de Gastropoda: análise em microscopia de varredura laser confocal
• Beneficiário: Toshie Kawano
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários