The timeline development of female canine germ cells

Autor(es)

de Souza, Aline F. ^[1] ; de Ramos, Eloise C. ^[1] ; Cury, Fabio S. ^[1] ; Pieri, Naira Caroline G. ^[2] ; Martins, Daniele S. ^{[3, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Av Duque de Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Reprod, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTION IN DOMESTIC ANIMALS; v. 54, n. 7, p. 964-971, JUL 2019.
• Citações Web of Science: 0

Resumo

During the sex differentiation, the primordial germ cells (PGCs) pass through a differentiation, becoming spermatogonial cells in males and oocytes in females. In this phase, there is difference in gene expression and differentiation potency between males and females. Specific cell markers have been essential in the PGC meiosis beginning and become oocyte cells. However, there are few studies about germline in domestic animals. The domestic dog (Canis lupus familiaris) is an interesting animal model to be used in the investigation about the mammal development because it has several biochemical and physiological similarities to humans. In addition, some additional investigations about dogs may contribute to a better understanding of the biology and genetic components, improving clinical veterinary and zoological sciences. Here, we elucidated by immunofluorescence and quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR), the dynamics of the expression of pluripotent (POU5F1 and NANOG) and germline (DDX4, DAZL and DPPA3) markers that are very important in the development of female canine germ cells during 35-50 days post-fertilization (dpf). The female canine germ cells were positive for pluripotent markers during middle developmental period. The number of DDX4, DAZL and DPPA3 cells increased along the germ cell maturation from 45 to 50 dpf. We provided an expression analysis of the pluripotent and germline markers in paraffin sections using the middle and later periods in female canine germ cells. The results can contribute the understanding about the timeline of each marker along the maturation of female canine germ cells. These results have a great significance to demonstrate the germ cell profile changes because it may allow the development of protocols about in vitro germ cell derivation. (AU)

• Processo FAPESP: 16/15260-6 - Identificação e rota de migração das células germinativas primordiais caninas
• Beneficiário: Eloise Cristina de Ramos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/10542-5 - Epigenética da reprogramação em células germinativas embrionárias caninas
• Beneficiário: Aline Fernanda de Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado