Short Cationic Peptide Derived from Archaea with Dual Antibacterial Properties and Anti-Infective Potential

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Candido, Elizabete S. ^{[1, 2]} ; Cardoso, Marlon H. ^{[3, 1, 2]} ; Chan, Lai Y. ^{[3, 4]} ; Torres, Marcelo D. T. ^{[5, 6, 7, 8, 9, 10, 11, 12, 13]} ; Oshiro, Karen G. N. ^{[2, 4]} ; Porto, William F. ^{[14, 2]} ; Ribeiro, Suzana M. ^[2] ; Haney, Evan F. ^[15] ; Hancock, Robert E. W. ^[15] ; Lu, Timothy K. ^{[7, 8, 9, 11, 12]} ; de la Fuente-Nunez, Cesar ^{[6, 7, 8, 9, 11, 12, 13]} ; Craik, David J. ^[3] ; Franco, Octavio L. ^{[1, 2, 4]}

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^[1] Univ Catolica Brasilia, Ctr Anal Prote & Bioquim, Posgrad Ciencias Genom & Biotecnol, SGAN 916 Modulo B, BR-70790160 Brasilia, DF - Brazil

^[2] Univ Catolica Dom Bosco, S Inova Biotech, Programa Posgrad Biotecnol, Ave Tamandare 6000, BR-79117900 Campo Grande, MG - Brazil

^[3] Univ Queensland, Inst Mol Biosci, 306 Carmody Rd, Brisbane, Qld 4072 - Australia

^[4] Univ Brasilia, Fac Med, Programa Posgrad Patol Mol, Campus Darcy Ribeiro, BR-70910900 Brasilia, DF - Brazil

^[5] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210170 Sao Paulo - Brazil

^[6] Univ Penn, Perelman Sch Med, Dept Psychiat, Philadelphia, PA 19104 - USA

^[7] MIT, Dept Elect Engn & Comp Sci, 77 Massachusetts Ave, Cambridge, MA 02139 - USA

^[8] Broad Inst MIT & Harvard, Cambridge, MA 02139 - USA

^[9] MIT, Ctr Microbiome Informat & Therapeut, 77 Massachusetts Ave, Cambridge, MA 02139 - USA

^[10] Univ Penn, Dept Bioengn, Philadelphia, PA 19104 - USA

^[11] MIT, MIT Synthet Biol Ctr, Synthet Biol Grp, 77 Massachusetts Ave, Cambridge, MA 02139 - USA

^[12] MIT, Elect Res Lab, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 - USA

^[13] Univ Penn, Perelman Sch Med, Dept Microbiol, Philadelphia, PA 19104 - USA

^[14] Porto Reports, BR-70790160 Brasilia, DF - Brazil

^[15] Univ British Columbia, Ctr Microbial Dis & Immun Res, Vancouver, BC V6T 1Z4 - Canada

• Tipo de documento: Artigo Científico
• Fonte: ACS INFECTIOUS DISEASES; v. 5, n. 7, p. 1081-1086, JUL 2019.
• Citações Web of Science: 2

Resumo

Bacterial biofilms and associated infections represent one of the biggest challenges in the clinic, and as an alternative to counter bacterial infections, antimicrobial peptides have attracted great attention in the past decade. Here, ten short cationic antimicrobial peptides were generated through a sliding-window strategy on the basis of the 19 amino acid residue peptide, derived from a Pyrobaculum aerophilum ribosomal protein. PaDBS1R6F10 exhibited anti infective potential as it decreased the bacterial burden in murine Pseudomonas aeruginosa cutaneous infections by more than 1000-fold. Adverse cytotoxic and hemolytic effects were not detected against mammalian cells. The peptide demonstrated structural plasticity in terms of its secondary structure in the different environments tested. PaDBS1R6F10 represents a promising antimicrobial agent against bacteria infections, without harming human cells. (AU)

• Processo FAPESP: 14/04507-5 - Aplicações biológicas de novos peptídeos antimicrobianos
• Beneficiário: Marcelo Der Torossian Torres
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/24413-0 - Peptídeos anfipáticos catiônicos antimicrobianos e antibiofilmes
• Beneficiário: Marcelo Der Torossian Torres
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado