P2X3 receptors contribute to muscle pain induced by static contraction by a mechanism dependent on neutrophil migration

Autor(es)

Aquino, Bruna de Melo ^[1] ; da Silva dos Santos, Diogo Francisco ^[1] ; Jorge, Carolina Ocanha ^[1] ; Salgado Marques, Aline Carolina ^[1] ; Teixeira, Juliana Maia ^[2] ; Parada, Carlos Amilcar ^[2] ; Goncalves Oliveira-Fusaro, Maria Claudia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Appl Sci, Lab Pain & Inflammat Res, Pedro Zaccaria 1300, Limeira, SP - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, Monteiro Lobato 255, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PURINERGIC SIGNALLING; v. 15, n. 2, p. 167-175, JUN 2019.
• Citações Web of Science: 1

Resumo

P2X3 receptors are involved with several pain conditions. Muscle pain induced by static contraction has an important socioeconomic impact. Here, we evaluated the involvement of P2X3 receptors on mechanical muscle hyperalgesia and neutrophil migration induced by static contraction in rats. Also, we evaluated whether static contraction would be able to increase muscle levels of TNF-alpha and IL-1 beta. Male Wistar rats were pretreated with the selective P2X3 receptor antagonist, A-317491, by intramuscular or intrathecal injection and the static contraction-induced mechanical muscle hyperalgesia was evaluated using the Randall-Selitto test. Neutrophil migration was evaluated by measurement of myeloperoxidase (MPO) kinetic-colorimetric assay and the cytokines TNF-alpha and IL-1 beta by enzyme-linked immunosorbent assay. Intramuscular or intrathecal pretreatment with A-317491 prevented static contraction-induced mechanical muscle hyperalgesia. In addition, A-317491 reduced static contraction-induced mechanical muscle hyperalgesia when administered 30 and 60min of the beginning of static contraction, but not after 30 and 60min of the end of static contraction. Intramuscular A-317491 also prevented static contraction-induced neutrophil migration. In a period of 24h, static contraction did not increase muscle levels of TNF-alpha and IL-1 beta. These findings demonstrated that mechanical muscle hyperalgesia and neutrophil migration induced by static contraction are modulated by P2X3 receptors expressed on the gastrocnemius muscle and spinal cord dorsal horn. Also, we suggest that P2X3 receptors are important to the development but not to maintenance of muscle hyperalgesia. Therefore, P2X3 receptors can be pointed out as a target to musculoskeletal pain conditions induced by daily or work-related activities. (AU)

• Processo FAPESP: 12/10402-6 - Envolvimento de mediadores inflamatórios e dos receptores P2X3 na hiperalgesia muscular induzida por contração isométrica sustentada em ratos
• Beneficiário: Bruna de Melo Aquino
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/23448-7 - Envolvimento de receptores do sistema nervoso central na hiperagesia muscular: papel dos receptores P2X3, AMPA e NMDA
• Beneficiário: Aline Carolina Salgado Marques
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 11/11064-4 - Desenvolvimento de um novo modelo de hiperalgesia muscular e estudo do envolvimento dos receptores P2X3 e p2X2/3 na hiperalgesia muscular
• Beneficiário: Maria Cláudia Gonçalves de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores