Salivary gland metabolism in an animal model of chronic kidney disease

Autor(es)

Carvalho, R. A. ^{[1, 2]} ; Romero, A. C. ^[3] ; Ibuki, F. K. ^[3] ; Nogueira, F. N. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Coimbra, Fac Sci & Technol, Dept Life Sci, Coimbra - Portugal

^[2] Univ Coimbra, Ctr Funct Ecol, Coimbra - Portugal

^[3] Univ Sao Paulo, Sch Dent, Dept Biomat & Oral Biol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 104, p. 40-45, AUG 2019.
• Citações Web of Science: 1

Resumo

Objective: The aim of this study was to determine the effects of experimental CKD into the metabolism of parotid and submandibular glands of rats. CKD was induced by 5/6 nephrectomy. Design: Serum analyses of BUN (Blood Urea Nitrogen) and creatinine concentrations were performed. Major salivary glands metabolism was investigated in vivo, both at rest and during salivary stimulation conditions by NMR isotopomer analysis, using {[}U-C-13]glucose as metabolic tracer. Results: CKD increases BUN and serum creatinine concentrations (p < 0.001). Multiple metabolic alterations were detected in the parotid glands of this animal model, including decreased concentrations of alanine (p < 0.05) and creatine (p < 0.05) and increased lactate/alanine ratios (p < 0.05). The salivary stimulus fostered accumulations of acetate at both analyzed glands of the CKD model (p < 0.05), indicative of disruption of the oxidative metabolic process. Conclusions: Experimental CKD induced by 5/6 nephrectomy altered the parotid salivary gland function, since glucose metabolism is clearly affected after stimulation for salivation in this gland. (AU)

• Processo FAPESP: 13/18609-1 - Dano oxidativo em glândulas salivares no diabete e na insuficiência renal crônica e possíveis benefícios da utilização de vitaminas C e E
• Beneficiário: Fernando Neves Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Regular